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Antioxidant Capacity and Antimutagenic Activity of Natural Oleoresin from Greenhouse Grown Tomatoes (Lycopersicon esculentum)

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Abstract

Natural oleoresins rich in lycopene were obtained from two varieties of tomato (Zedona and Gironda) and their nutraceutical potential (antioxidant and antimutagenic capacity) was evaluated. Both oleoresins had a high content of lycopene, 58.33 ± 1.67 mg/g (Zedona) and 63.97 ± 0.80 mg/g (Gironda). The antioxidant activity (AA) of the oleoresins by β-carotene method were 56.4–74.5% (Zedona) and 51–72.8% (Gironda), while when using the free radical stable 2,2-diphenyl-picryl-hydrazyl (DPPH) method, the antiradical activity (ARA) was determined to be 18.2–32.7% (Zedona) and 16.6–26.7% (Gironda) for the concentrations tested that of 200–400 μM equivalents of lycopene. The antimutagenic activity of the oleoresins was tested against aflatoxin B1 (AFB1) using the microsuspension assay, both varieties had a very high antimutagenic potential against AFB1 (60–66%).These results suggest the NCRT can be taken advantage to obtaining rich oleoresin in lycopene with a nutraceutical value.

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Abbreviations

NCRT:

Non commercial red tomato

AA:

Antioxidant activity

DPPH:

2,2-diphenyl-2-picrylhydrazyl

ARA:

Antiradical activity

AFB1 :

Aflatoxin B1

BHT:

Butylated hydroxytoluene

ADIBA:

α, α′-Azodiisobutyramidine dihydrochloride

DMSO:

Dimethyl sulfoxide

References

  1. Instituto Nacional de Estadística y Geografía México

  2. La Vecchia C, Altieri A, Tavani A (2001) Vegetables, fruit, antioxidants and cancer: a review of Italian studies. Eur J Nutr 40:261–267. doi:10.1007/s394-001-8354-9

    Article  Google Scholar 

  3. Willcox JK, Catignani GL, Lazarus S (2003) Tomatoes and cardiovascular health. Crit Rev Food Sci Nutr 43:1–18. doi:10.1080/10408690390826437

    Article  CAS  Google Scholar 

  4. Johnson EJ (2002) The role of carotenoids in human health. Nutr Clin Care 5:56–65. doi:10.1046/j.1523-5408.2002.00004.x

    Article  Google Scholar 

  5. Cantrell A, McGarvey DJ, Truscott TG, Rancan F, Bohm F (2003) Single oxygen quenching by dietary carotenoids in a model membrane environment. Arch Biochem Biophys 412:47–54. doi:10.1016/S0003-9861(03)00014-6

    Article  CAS  Google Scholar 

  6. Fraser ML, Lee AH, Binns CW (2005) Lycopene and prostate cancer: emerging evidence. Expert Rev Anticancer Ther 5:847–854. doi:10.1586/14737140.5.5.847

    Article  CAS  Google Scholar 

  7. Kubota C, Thomson CA, Wu M, Javanmardi J (2006) Controlled environments for production of value-added food crops with high phytochemical concentrations: lycopene in tomato as an example. HortScience 41:522–525

    CAS  Google Scholar 

  8. López-Camelo AF, Gómez PA (2004) Comparison of color indexes for tomato ripening. Hortic Bras 22:534–537. doi:10.1590/S0102-05362004000300006

    Article  Google Scholar 

  9. McLaren K (1980) Food colorimetry. In: Walford J (eds) Developments in food colours I. Applied Science, London, pp 27–45

    Google Scholar 

  10. Arias R, Lee TC, Logendra L, Janes H (2000) Correlation of lycopene measured by HPLC with the L*, a*, b* color readings of a hydroponic tomato and the relationship of maturity with color and lycopene content. J Agric Food Chem 48:1697–1702. doi:10.1021/jf990974e

    Article  CAS  Google Scholar 

  11. Fukumoto LR, Mazza G (2000) Assessing antioxidant and prooxidant activities of phenolic compounds. J Agric Food Chem 48:3597–3604. doi:10.1021/jf000220w

    Article  CAS  Google Scholar 

  12. Marco GJ (1968) A rapid method for evaluation of antioxidants. J Am Oil Chem Soc 45:594–598. doi:10.1007/BF02668958

    Article  CAS  Google Scholar 

  13. Velioglu YS, Mazza G, Gao L, Oomah BD (1997) Antioxidant activity and total phenolics in selected fruits, vegetables and grains products. J Agric Food Chem 45:304–309. doi:10.1021/jf960421t

    Article  Google Scholar 

  14. Burda S, Oleszek W (2001) Antioxidant and antiradical activities of flavonoids. J Agric Food Chem 49:2774–2779. doi:10.1021/jf001413m

    Article  CAS  Google Scholar 

  15. Kado NY, Langley D, Eisenstadt EA (1983) A simple modification of the Salmonella liquid incubation assay. Increased sensitivity for detecting mutagens in human urine. Mutat Res 121:25–32. doi:10.1016/0165-7992(83)90082-9

    Article  CAS  Google Scholar 

  16. Kado NY, Guirguis GN, Flessel CP, Chan RC, Chang K, Wesolowski JJ (1986) Mutagenicity of fine (<2.5 μm) airbone particles: diurnal variation in community air determined by a Salmonella micro preincubation (microsuspension) procedure. Environ Mutagen 8:53–56. doi:10.1002/em.2860080106

    Article  CAS  Google Scholar 

  17. Ames BN, McCann J, Yamasaki E (1975) Methods for detecting carcinogens and mutagens with the salmonella/mammalian microsome mutagenicity test. Mutat Res 31:347–364

    CAS  Google Scholar 

  18. SAS Institute (1999) SAS user’s guide: statistics, 8th edn. SAS Institute, Cary

    Google Scholar 

  19. Shewfelt R, Thai C, Davis J (1988) Prediction of changes in color of tomatoes during ripening at different constant temperature. J Food Sci 53:1433–1437. doi:10.1111/j.1365-2621.1988.tb09293.x

    Article  Google Scholar 

  20. Clément A, Dorais M, Vernon M (2008) Multivariate approach to the measurement of tomato maturity and gustatory attributes and their rapid assessment by Vis-NIR Spectroscopy. J Agric Food Chem 56:1538–1544. doi:10.1021/jf072182n

    Article  Google Scholar 

  21. Thompson KA, Marshall MR, Sims CA, Wei CI, Sargent SA, Scott JW (2000) Cultivar, maturity and heat treatment on lycopene content in tomatoes. J Food Sci 65:791–795. doi:10.1111/j.1365-2621.2000.tb13588.x

    Article  CAS  Google Scholar 

  22. Toor RK, Lister CE, Savage GP (2005) Antioxidant activities of New Zealand-grown tomatoes. Int J Food Sci Nutr 56:597–605. doi:10.1080/09637480500490400

    Article  CAS  Google Scholar 

  23. Leonardi C, Ambrosino P, Esposito F, Fogliano V (2000) Antioxidative activity and carotenoid and tomatine contents in different typologies of fresh consumption tomatoes. J Agric Food Chem 48:4723–4727. doi:10.1021/jf000225t

    Article  CAS  Google Scholar 

  24. Lavelli V, Peri C, Rizzolo A (2000) Antioxidant activity of tomato products as studied by model reactions using xanthine oxidase, myeloperoxidase, and copper-induced lipid peroxidation. J Agric Food Chem 48:1442–1448. doi:10.1021/jf990782j

    Article  CAS  Google Scholar 

  25. van Boekel MAJS, Jongen WMF (1997) Product quality and food processing: how to quantify the healthiness of a product. Cancer Lett 114:65–69. doi:10.1016/S0304-3835(97)04627-2

    Article  Google Scholar 

  26. Stahl W, Junghans A, de Boer B, Driomina ES, Briviba K, Sies H (1998) Carotenoid mixtures protect multilamellar liposomes against oxidative damage: synergistic effects of lycopene and lutein. FEBS Lett 427:305–308. doi:10.1016/S0014-5793(98)00434-7

    Article  CAS  Google Scholar 

  27. Matulka RA, Hood AM, Griffiths JC (2004) Safety evaluation of a natural tomato oleoresin extract derived from food-processing tomatoes. Regul Toxicol Pharmacol 39:390–402. doi:10.1016/j.yrtph.2004.03.005

    Article  CAS  Google Scholar 

  28. Weisburger JH, Dolan L, Pittman B (1998) Inhibition of PhIP mutagenicity by caffeine, lycopene, daidzein, and genistein. Mutat Res 416:125–128

    CAS  Google Scholar 

  29. Rauscher R, Edenharder R, Platt KL (1998) In vitro antimutagenic and in vivo anticlastogenic effects of carotenoids and solvent extracts from fruits and vegetables rich in carotenoids. Mutat Res 413:129–142

    CAS  Google Scholar 

  30. Campbell JK, Canene-Adams K, Lindshield BL, Boileau TWM, Clinton SK, Erdman JW (2004) Tomato phytochemicals and prostate cancer risk. J Nutr 134:3486S–3492S

    CAS  Google Scholar 

  31. Jian L, Du CJ, Lee AH, Binns CW (2005) Do dietary lycopene and other carotenoids protect against prostate cancer? Int J Cancer 113:1010–1014. doi:10.1002/ijc.20667

    Article  Google Scholar 

  32. Stahl W, Heinrich U, Aust O, Tronnier H, Sies H (2006) Lycopene-rich products and dietary photoprotection. Photochem Photobiol Sci 5:238–242. doi:10.1039/b505312a

    Article  CAS  Google Scholar 

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Acknowledgements

The authors wish to thank SIHGO program from Consejo Nacional de Ciencia y Tecnología (CONACYT), for the financial support granted for the accomplishment of the present work. They also thank Silvia C. Stroet for providing editorial services from UAQ’s Translation and Edition Office.

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Authors and Affiliations

  1. Centro de Estudios Académicos sobre Contaminación Ambiental, Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, Qro, 76010, México

    Eustolia Rodríguez-Muñoz & Gustavo Pedraza-Aboytes

  2. Facultad de Ingeniería, Universidad Autónoma de Querétaro, Querétaro, Qro, 76010, México

    Gilberto Herrera-Ruiz

  3. Programa de Posgrado en Alimentos del Centro de la República (PROPAC), Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, Qro, 76010, México

    Guadalupe Loarca-Piña

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  1. Eustolia Rodríguez-Muñoz
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  2. Gilberto Herrera-Ruiz
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  3. Gustavo Pedraza-Aboytes
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  4. Guadalupe Loarca-Piña
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Correspondence to Guadalupe Loarca-Piña.

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Rodríguez-Muñoz, E., Herrera-Ruiz, G., Pedraza-Aboytes, G. et al. Antioxidant Capacity and Antimutagenic Activity of Natural Oleoresin from Greenhouse Grown Tomatoes (Lycopersicon esculentum). Plant Foods Hum Nutr 64, 46–51 (2009). https://doi.org/10.1007/s11130-008-0099-3

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