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Impact of elevated temperatures on specific leaf weight, stomatal density, photosynthesis and chlorophyll fluorescence in soybean

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Abstract

High-temperature stress is a major environmental stress and there are limited studies elucidating its impact on soybean (Glycine max L. Merril.). The objectives of present study were to quantify the effect of high temperature on changes in leaf thickness, number of stomata on adaxial and abaxial leaf surfaces, gas exchange, chlorophyll fluorescence parameters and seed yield in soybean. Twelve soybean genotypes were grown at day/night temperatures of 30/22, 34/24, 38/26 and 42/28 °C with an average temperature of 26, 29, 32 and 35 °C, respectively, under greenhouse conditions. One set was also grown under ambient temperature conditions where crop season average maximum, minimum and mean temperatures were 28.0, 22.4 and 25.2 °C, respectively. Significant negative effect of temperature was observed on specific leaf weight (SLW) and leaf thickness. Rate of photosynthesis, stomatal conductance and water use efficiency declined as the growing temperatures increased; whereas, intercellular CO2 and transpiration rate were increased. With the increase in temperature chlorophyll fluorescence parameters such as Fv/Fm, qP and PhiPSII declined while there was increase in qN. Number of stomata on both abaxial and adaxial surface of leaf increased significantly with increase in temperatures. The rate of photosynthesis, PhiPSII, qP and SPAD values were positively associated with leaf thickness and SLW. This indicated that reduction in photosynthesis and associated parameters appears to be due to structural changes observed at higher temperatures. The average seed yield was maximum (13.2 g/pl) in plants grown under ambient temperature condition and declined by 8, 14, 51 and 65% as the temperature was increased to 30/22, 34/24, 38/26 and 42/28 °C, respectively.

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Acknowledgements

Kanchan Jumrani would like to acknowledge the Council of Scientific and Industrial Research (CSIR)/University Grants commission (UGC), Government of India (20-06/2010 (i) EU-IV) for providing the financial support in the form of Research Fellowship.

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Authors and Affiliations

  1. School of Life Sciences, DAVV, Khandwa Road, Indore, 452 001, India

    Kanchan Jumrani & Govind Prakash Pandey

  2. Directorate of Soybean Research, Khandwa Road, Indore, 452 001, India

    Virender Singh Bhatia

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  1. Kanchan Jumrani
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  2. Virender Singh Bhatia
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  3. Govind Prakash Pandey
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Correspondence to Kanchan Jumrani.

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Jumrani, K., Bhatia, V.S. & Pandey, G.P. Impact of elevated temperatures on specific leaf weight, stomatal density, photosynthesis and chlorophyll fluorescence in soybean. Photosynth Res 131, 333–350 (2017). https://doi.org/10.1007/s11120-016-0326-y

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