Abstract
Plant stems are involved in supporting the entire plant body, thus having an important effect on the yield of oilseed rape. The current understanding of the mechanism of stem development in oilseed rape is limited. In this study, a detailed comparative analysis of mRNA expression levels in Brassica napus between strong- (sample numbers 7110, 7031, 7029, 7095, and 7109) and weak-stemmed (sample numbers 7003, 7088, and 7123) was performed using RNA sequencing. A total of 1531 significantly differentially expressed genes were identified, including genes involved in cellulose and lignin biosynthesis, cell wall-binding proteins, and hormone biosynthesis and response genes. These results provide valuable insights into the molecular basis of stem development at the transcriptional level and will be helpful for revealing the regulatory mechanisms of stem growth in B. napus.
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We gratefully acknowledge the National Natural Science Foundation of China (grant number 31671733), the National Grand Project of Science and Technology (grant number 2018ZX0801104B), the Breeding Special Grants of Seven Major Crops (2017YFD0102000), and the Major Research Project of CAAS Science and Technology Innovation Program supported this study.
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XY and GW conceived the study and supervised the project. RY and XZ performed the experiments and wrote the paper. SZ analyzed the data. All authors edited and approved the final manuscript.
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Yuan, R., Zeng, X., Zhao, S. et al. Identification of Candidate Genes Related to Stem Development in Brassica napus Using RNA-Seq. Plant Mol Biol Rep 37, 347–364 (2019). https://doi.org/10.1007/s11105-019-01158-1
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DOI: https://doi.org/10.1007/s11105-019-01158-1