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Chlorophyll deficient 3, Encoding a Putative Potassium Efflux Antiporter, Affects Chloroplast Development Under High Temperature Conditions in Rice (Oryza sativa L.)

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Abstract

Potassium efflux antiporters (KEAs) play important roles in the regulation of monovalent cation efflux to maintain ion homeostasis in plant cells; however, details of their function in chloroplast development remain elusive, especially under stress conditions. Here, we identified a KEA family gene based on three chlorophyll-deficient mutants, cde3-1, 2, 3. These cde3 mutants exhibit serious white stripe leaf and an albino midrib phenotype, reduced level of photosynthetic pigments, and arrested chloroplast development under high-temperature conditions. Map-based cloning and allelism tests showed that CDE3 encodes a putative KEA protein in rice. CDE3 is mainly expressed in green tissues, with maximum transcript abundance in young leaves. Subcellular localization analysis showed that CDE3 is localized in chloroplasts. Quantitative real-time PCR analysis showed that expression levels of genes associated with chlorophyll biosynthesis, photosynthesis, and chloroplast development are affected in cde3-1, which suggest that CDE3 plays an important role in chloroplast K+/H+ homeostasis and chloroplast development under high-temperature conditions.

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Funding

This work was supported by the National Key Research and Development Program of China (2016YFD0101801), the National Natural Science Foundation of China (31521064), and by the National S&T Major Project (2016ZX08001006).

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Author notes

  1. Rongjian Luo and Hanwei Jiang contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, People’s Republic of China

    Rongjian Luo, Hanwei Jiang, Yusong Lv, Shikai Hu, Zhonghua Sheng, Gaoneng Shao, Shaoqing Tang, Peisong Hu & Xiangjin Wei

  2. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 311121, People’s Republic of China

    Hanwei Jiang

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  1. Rongjian Luo
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Contributions

X. W. and P. H. designed the experiments. R. L., H. J., X. W., Z. S., and G. S. carried out the experimental work. H. J. and X. W. wrote the first draft of the manuscript which was critically revised and implemented by Y. L., S. H., and S. T. All authors discussed the results and commented on the final version of the manuscript.

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Correspondence to Xiangjin Wei.

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Luo, R., Jiang, H., Lv, Y. et al. Chlorophyll deficient 3, Encoding a Putative Potassium Efflux Antiporter, Affects Chloroplast Development Under High Temperature Conditions in Rice (Oryza sativa L.). Plant Mol Biol Rep 36, 675–684 (2018). https://doi.org/10.1007/s11105-018-1109-8

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