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Different Gene Expressions of Resistant and Susceptible Maize Inbreds in Response to Fusarium verticillioides Infection

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Abstract

Ear rot, caused by Fusarium verticillioides (FV), is a destructive disease of maize as it reduces grain yield and increases risks of mycotoxin production, thus endangering livestock. To identify genes differentially expressed during FV infection, four cDNA libraries were constructed for suppression subtractive hybridization using RNA isolated from bracts of an FV-resistant inbred maize line, Bt-1, as well as an FV-susceptible maize inbred line, Ye478. A total of 145 clones were obtained following reverse dot-blot hybridization, and these were sequenced from these libraries. Similarity analysis revealed that of these genes, 93 were unique, including 68 of known function, 24 of unknown function, and a single novel gene. Most genes of known function were predominantly involved in plant defense such as cell defense, transcription regulation, signal transduction, and metabolism. Expression profiles of eight representative genes, using semiquantitative reverse transcription-polymerase chain reaction, confirmed that differential gene regulation during FV infection was involved. These findings suggested that these differentially expressed genes might be involved in FV defense responses in maize.

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Acknowledgments

We thank Dr. Yunbi Xu and Dr. Xiaoyang Zhu for their comments on this manuscript. This project was financially supported by grants from the Natural National Science Foundation of China (nos. 30571173 and 31201274), Program for Changjiang Scholars and Innovative Research Team in University of China (grant no. IRT0453), and National High Technology Research and Development Program of China (863 Program) (no. 2012AA10A307).

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  1. Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Maize Research Institute, Sichuan Agricultural University, Ya’an, 625014, People’s Republic of China

    Guangsheng Yuan, Zhiming Zhang, Kui Xiang, Yaou Shen, Juan Du, Haijian Lin, Li Liu, Maojun Zhao & Guangtang Pan

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  1. Guangsheng Yuan
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  2. Zhiming Zhang
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Correspondence to Guangtang Pan.

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Yuan, G., Zhang, Z., Xiang, K. et al. Different Gene Expressions of Resistant and Susceptible Maize Inbreds in Response to Fusarium verticillioides Infection. Plant Mol Biol Rep 31, 925–935 (2013). https://doi.org/10.1007/s11105-013-0567-2

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