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QTL mapping of root aerenchyma formation in seedlings of a maize × rare teosinte “Zea nicaraguensis” cross

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Abstract

Using a 141 F2 population generated from maize inbred B64 × teosinte Zea nicaraguensis cross, quantitative trait loci (QTLs) controlling aerenchyma formation in roots under non-flooding drained soil conditions were identified. Seedlings of Z. nicaraguensis formed clear aerenchyma in the cortex of adventitious roots in non-flooding conditions, whereas the maize inbred line B64 did not. In the F2 population, the capacity to develop aerenchyma exhibited wide and continuous variation, suggesting the trait was controlled by multiple genes. A linkage map was developed using 85 SSR markers, covering 1,224 cM across all ten chromosomes. Composite interval mapping analysis revealed that four QTLs for aerenchyma formation under non-flooding conditions were located to two regions of chromosome 1 (identified as Qaer1.02-3 and Qaer1.07), chromosome 5 (Qaer5.09) and chromosome 8 (Qaer8.06-7), and these explained 46.5% of the total phenotypic variance. The multiple interval mapping approach identified additional QTLs on chromosomes 1 (Qaer1.01) and 5 (Qaer5.01). Using these results, it may be possible to use SSR markers linked to aerenchyma formation in a marker assisted selection approach to introduce aerenchyma formation in drained soil conditions into maize for the eventual development of flooding tolerant maize hybrids.

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Acknowledgments

We are grateful to the International Maize and Wheat Improvement Center (CIMMYT), Mexico for providing seed of Z. nicaraguensis, the Gene Bank, National Institute of Agrobiological Sciences, Tsukuba, Japan for supplying seed of B64, and the Corn and Sorghum Breeding laboratory, National Institute of Livestock and Grassland Science, Nasushiobara, Japan for supporting this work. We thank Dr. K. Takeda (Research Institute for Bioresources, Okayama University, Kurashiki, Japan), Dr. T. Komatsuda (National Institute of Agrobiological Sciences, Tsukuba, Japan), and Dr. M. Nakazono (University of Tokyo, Bunkyo-ku, Japan) for critically reviewing the manuscript.

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Authors and Affiliations

  1. Department of Forage Crop Breeding, National Institute of Livestock and Grassland Science, Nasushiobara, Tochigi, 329-2793, Japan

    Y. Mano, F. Omori & T. Takamizo

  2. USDA-ARS Grazinglands Research Laboratory, 7207 West Cheyenne St, El Reno, OK, 73036, USA

    B. Kindiger

  3. Department of Crop Science, North Carolina State University, Raleigh, NC, 27695-7620, USA

    R. McK. Bird

  4. REGEN-FAGRO, Universidad Nacional Agraria, Kilómetro 12.5 Carretera Norte, Managua, Nicaragua

    C. H. Loaisiga

  5. Faculty of Bioresource Sciences, Akita Prefectural University, Akita City, Akita, 010-0195, Japan

    H. Takahashi

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  1. Y. Mano
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Correspondence to Y. Mano.

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Mano, Y., Omori, F., Takamizo, T. et al. QTL mapping of root aerenchyma formation in seedlings of a maize × rare teosinte “Zea nicaraguensis” cross. Plant Soil 295, 103–113 (2007). https://doi.org/10.1007/s11104-007-9266-9

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