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Genetic Diversity of Rhizobia Nodulating Arachis hypogaea L. in Central Argentinean Soils

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Abstract

The diversity of thirty-nine isolates from peanut plants growing at fourteen different sites in the Argentinean province of Córdoba was examined by rep-PCR, RFLP of PCR amplified 16S rRNA gene and complete sequencing of ribosomal genes. The genomic analysis of the peanut isolates indicated that each group encompasses heterogeneity among their members, having distinct rep fingerprints and 16S rRNA alleles. Complete sequencing of 16S rRNA demonstrated that native peanut rhizobia from Córdoba soils representative of the slow and fast growers are phylogenetically related to Bradyrhizobium japonicum and Bradyrhizobium sp. and Rhizobium giardinii and R. tropici species, respectively. The nodC gene sequence analysis showed phylogenetic similarity between fast grower peanut symbionts and Rhizobium tropici.

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References

  • O M Aguilar M V López P M Riccillo R A Gonzales M Pagano D H Grasso A Pühler G Favelukes (1998) ArticleTitlePrevalence of the Rhizobium etli-like allele in genes coding for 16S rRNA among the indigenous rhizobial populations found associated with wild beans from the Southern Andes in Argentina Appl. Environ. Microbiol. 64 3520–3524 Occurrence Handle9726909 Occurrence Handle1:CAS:528:DyaK1cXmtVajtbg%3D

    PubMed  CAS  Google Scholar 

  • Altschul S, Madden T, Schäffer A, Zhang J, Zhang Z, Miller W and Lipman D 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search.

  • N Alwi J C Wynne J O Rawlings T J Schneewies G H Elkan (1989) ArticleTitleSymbiotic Relationship between Bradyrhizobium strains and peanut Crop Sci. 29 50–55 Occurrence Handle10.2135/cropsci1989.0011183X002900010011x

    Article  Google Scholar 

  • N Amarger (2001) ArticleTitleRhizobia in the field Advances in Agronomy 73 109–168 Occurrence Handle1:CAS:528:DC%2BD3MXjs1artb8%3D Occurrence Handle10.1016/S0065-2113(01)73006-4

    Article  CAS  Google Scholar 

  • N Amarger V Macheret G Laguerre (1997) ArticleTitleRhizobium gallicum sp. Nov. And Rhizobium giardinii sp. Nov. from Phaseolus vulgaris nodules Int. J. Syst. Bacteriol. 47 996–1006 Occurrence Handle9336898 Occurrence Handle1:STN:280:DyaK2svnsl2rtw%3D%3D

    PubMed  CAS  Google Scholar 

  • Y Benhinzia H Benhinzia A Benguedouar R Muresu A Giacomini A Squartini (2004) ArticleTitleGamma Proteobacteria Can Nodulate Legumes of the Genus Hedysarum Syst. Appl. Microbiol. 27 462–468 Occurrence Handle10.1078/0723202041438527

    Article  Google Scholar 

  • E S P Bromfield L R Barran R Wheatcroft (1995) ArticleTitleRelative genetic structure of a population of Rhizobium meliloti isolated directly from soil and from nodules of alfalfa (Medicago sativa) and sweet clover (Melilotus alba) Mol. Ecol. 4 183–188

    Google Scholar 

  • W M Chen L Moulin C Bontemps P Vandamme G Béna C Boivin-Masson (2003) ArticleTitleLegume symbiotic nitrogen fixation by β-proteobacteria is widespread in nature J. Bacteriol. 185 7266–7272 Occurrence Handle14645288 Occurrence Handle1:CAS:528:DC%2BD3sXpvVaqtro%3D Occurrence Handle10.1128/JB.185.24.7266-7272.2003

    Article  PubMed  CAS  Google Scholar 

  • W X Chen Z Y Tan J L Gao Y Li E T Wang (1997) ArticleTitleRhizobium hiananense sp. nov., isolated from tropical legumes Int. J. Syst. Bacteriol. 67 3677–3682

    Google Scholar 

  • L M O Chueire Particlede M Hungría (1997) ArticleTitleN2-fixation ability of Brazilian soybean cultivars with Sinorhizobium fredii and Sinorhizobium xinjiangense Plant and Soil 196 1–5 Occurrence Handle10.1023/A:1004222218007

    Article  Google Scholar 

  • F Doignon-Bourcier A Willems R Coopman G Laguerre M Gillis P Lajudie Particlede (2000) ArticleTitleGenotypic characterization of Bradyrhizobium strains nodulating small Senegalese legumes by 16S-23S rRNA intergenic gene spacers and amplified fragment length polymorphism fingerprint analyses Appl. Environ. Microbiol. 66 3987–3997 Occurrence Handle10966419 Occurrence Handle1:CAS:528:DC%2BD3cXmsVajsL0%3D Occurrence Handle10.1128/AEM.66.9.3987-3997.2000

    Article  PubMed  CAS  Google Scholar 

  • L Frioni A Rodriguez M Meerhoff (2001) ArticleTitleDifferentiation of rhizobia isolated from native legumes trees in Uruguay Appl. Soil Ecol. 16 275–282 Occurrence Handle10.1016/S0929-1393(00)00124-4

    Article  Google Scholar 

  • W K Gillette G H Elkan (1996) ArticleTitleBradyrhizobium (Arachis) sp. strain NC92 contains two nodD genes involved in the repression of nodA and nolA gene required for the efficient nodulation of host plants J. Bacteriol. 178 2757–2766 Occurrence Handle8631662 Occurrence Handle1:CAS:528:DyaK28XivFGktb0%3D

    PubMed  CAS  Google Scholar 

  • P H Graham M J Sadowsky H H Keyser Y M Barnet R S Bradley J E Cooper J Ley ParticleDe B D W Jarvis E B Roslycky B W Strijdom J P W Young (1991) ArticleTitleProposed minimal standards for the description of new genera and species of root- and stem-nodulating bacteria Int. J. Syst. Bacteriol. 41 582–587J

    Google Scholar 

  • Haukka K 1997 Genetic diversity and phylogeny of rhizobia isolated from tropical tree legumes. In Dissertations Biocentri Viikki Universitatis Helsingiensis. PhD thesis. Department of Applied Chemistry and Microbiology, University of Helsinki.

  • P R Hirsch (1996) ArticleTitlePopulations dynamics of indigenous and genetically modified rhizobia in the field New Phytol. 133 159–171 Occurrence Handle10.1111/j.1469-8137.1996.tb04351.x

    Article  Google Scholar 

  • P Irisarri F Milnitsky J Monza E Bedmar (1996) ArticleTitleCharacterization of rhizobia nodulating Lotus subbiflorus from Uruguayan soils Plant and Soil 180 39–47 Occurrence Handle1:CAS:528:DyaK28XltFWgsLo%3D Occurrence Handle10.1007/BF00015409

    Article  CAS  Google Scholar 

  • B D Jarvis P Berkum Particlevan W X Chen S M Nour M P Fernandez J C Cleyet-Marel M Gillis (1997) ArticleTitleTransfer of Rhizobium loti, Rhizobium huaukii, Rhizobium ciceri, Rhizobium mediterraneum and Rhizobium thiashanense to Mesorhizobium genus Nov. Int. J. Syst. Bacteriol. 47 895–898

    Google Scholar 

  • D C Jordan (1984) Rhizobiaceae Conn N R. Krieg J G Holt (Eds) Bergeýs Manual of Systematic Bacteriology Williams and Wilkins Baltimore, Md. USA 235–244

    Google Scholar 

  • G Kochert H T Stalker M Gimenes L Galaro C R Lopes K Moore (1996) ArticleTitleRFLP, cytogenetic evidence on the origin and evolution of allotetraploid domesticated peanut, A. hypogaea (Leguminosae) Am. J. Bot. 83 1282–1991 Occurrence Handle1:CAS:528:DyaK28XmvF2isr8%3D Occurrence Handle10.2307/2446112

    Article  CAS  Google Scholar 

  • A Krapovickas (1969) The origin variability and spread of the groundnut (Arachis hypogaea) P J Ucko I S Falk (Eds) The Domestication and Exploitation of Plants and Animals Gerald Duckworth London 427–441

    Google Scholar 

  • G Laguerre M R Allard F Revoy N Amarger (1994) ArticleTitleRapid identification of rhizobia by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes Appl. Environ. Microbiol. 60 56–63 Occurrence Handle16349165 Occurrence Handle1:CAS:528:DyaK2cXntlaksQ%3D%3D

    PubMed  CAS  Google Scholar 

  • G Laguerre S M Nour V Macheret J Sanjuan P Drouin N Amarger (2001) ArticleTitleClassification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts Microbiol. 147 981–993 Occurrence Handle1:CAS:528:DC%2BD3MXjtVeisb8%3D

    CAS  Google Scholar 

  • N Marsudi A Glenn M Dilworth (1999) ArticleTitleIdentification and characterization of fast- and slow- growing root nodule bacteria from South-Western Australian soils able to nodulate Acacia saligna Soil Biol. Biochem. 31 1229–1238 Occurrence Handle1:CAS:528:DyaK1MXktlyjurg%3D Occurrence Handle10.1016/S0038-0717(99)00032-2

    Article  CAS  Google Scholar 

  • E Martínez-Romero J Caballero-Mellado (1996) ArticleTitleRhizobium Phylogenies and Bacterial Genetic Diversity Crit. Rev. Plant Sci. 15 113–140

    Google Scholar 

  • E Martínez-Romero E Segovia F M Mercante A A Franco P H Graham M A Pardo (1991) ArticleTitleRhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees Int. J. Syst. Bacteriol. 41 417–426 Occurrence Handle1715738

    PubMed  Google Scholar 

  • H Meade S Long G Ruvkun S Brown F Ausubel (1982) ArticleTitlePhysical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti mutants induced by Tn5 mutagenesis J. Bacteriol. 149 114–122 Occurrence Handle6274841 Occurrence Handle1:CAS:528:DyaL38XpvVKitg%3D%3D

    PubMed  CAS  Google Scholar 

  • L Moulin A Munive B Dreyfus C Boivin-Masson (2001) ArticleTitleNodulation of legumes by members of β-subclass of Proteobacteria Nature 411 948–950 Occurrence Handle11418858 Occurrence Handle1:CAS:528:DC%2BD3MXkvVWhu7Y%3D Occurrence Handle10.1038/35082070

    Article  PubMed  CAS  Google Scholar 

  • D W Odee J M Sutherland E T Makatiani S G McInroy J I Sprent (1997) ArticleTitlePhenotypic characteristics and composition of rhizobia associated with woody legumes growing in diverse Kenyan conditions Plant and Soil 188 65–75 Occurrence Handle1:CAS:528:DyaK2sXjvFyjtL4%3D Occurrence Handle10.1023/A:1004204413140

    Article  CAS  Google Scholar 

  • H Oyaizu S Matsumoto K Minamisawa T Gamou (1993) ArticleTitleDistribution of rhizobia in leguminous plants as surveyed by phylogenetic identification J. Gen. Appl. Microbiol. 39 339–354 Occurrence Handle1:CAS:528:DyaK2cXhvVOjt7o%3D

    CAS  Google Scholar 

  • S G Pueppke W J Broughton (1999) ArticleTitleRhizobium sp Strain NGR234 and R. fredii USDA257 Share Exceptionally Broad, Nested Host Ranges Mol. Plant-Microbe Interact. 12 293–318 Occurrence Handle10188270 Occurrence Handle1:CAS:528:DyaK1MXisVCgsrg%3D

    PubMed  CAS  Google Scholar 

  • Sambrook J, Fritsch E F and Maniatis T 1989 Molecular Cloning: A Laboratory Manual, 2nd ed.: Cold Spring Harbor Laboratory Cold Spring Harbor, NY.

  • M Santamaria J Corzo M León-Barrios A Gutiérrez-Navarro (1997) ArticleTitleCharacterisation and differentiation of indigenous rhizobia isolated from Canarian shrub legumes of agricultural and ecological interest Plant and Soil 190 143–152 Occurrence Handle1:CAS:528:DyaK2sXkvFyjur8%3D Occurrence Handle10.1023/A:1004247803116

    Article  CAS  Google Scholar 

  • T Taurian O M Aguilar A Fabra (2002) ArticleTitleCharacterization of nodulating peanut rhizobia isolated from a native soil population in Córdoba, Argentina Symbiosis 25 49–53

    Google Scholar 

  • J Thompson T Gibson F Plewniak F Jeanmougin D Higgins (1997) ArticleTitleThe CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Nucl. Acids Res. 25 4876–4882 Occurrence Handle9396791 Occurrence Handle1:CAS:528:DyaK1cXntFyntQ%3D%3D Occurrence Handle10.1093/nar/25.24.4876

    Article  PubMed  CAS  Google Scholar 

  • M E Trujillo A Willems A Abril A -M Planchuelo R Rivas D Ludeña P F Mateos E Martínez-Molina E Velázquez (2005) ArticleTitleNodulation of Lupinus albus by Strains of Ochrobactrum lupini sp. nov Appl. Env. Microbiol. 71 1318–1327 Occurrence Handle1:CAS:528:DC%2BD2MXisVOis70%3D Occurrence Handle10.1128/AEM.71.3.1318-1327.2005

    Article  CAS  Google Scholar 

  • B E Urtz G H Elkan (1996) ArticleTitleGenetic diversity among Bradyrhizobium isolates that effectively nodulate peanut (Arachis hypogaea) Can. J. Microbiol. 188 65–75

    Google Scholar 

  • P Berkum Particlevan J J Fuhrman (2000) ArticleTitleEvolutionary relationships among the soybean bradyrhizobia reconstructed from 16S rRNA gene and internally transcribed spacer region sequence divergence Int. J. Syst. Evol. Microbiol. 50 2165–2172 Occurrence Handle11155993

    PubMed  Google Scholar 

  • P Berkum Particlevan D Beyene G P Bao T A Campell B D Eardly (1998) ArticleTitleRhizobium mongolense sp. nov. is one of three rhizobial genotypes identified which nodulate and form nitrogen-fixing symbiosis with Medicago ruthenica ((L.) Ladebour) Int. J. Syst. Bacteriol. 48 13–22 Occurrence Handle9542071 Occurrence Handle10.1099/00207713-48-1-13

    Article  PubMed  Google Scholar 

  • D Rossum Particlevan F P Schuurmans M Gillis A M Muyotcha H W Verseveld Particlevan A H Stouthhamer F C Boogerd (1995) ArticleTitleGenetic and phenetic analyses of Bradyrhizobium strains nodulating peanut (Arachis hypogaea) roots Appl. Environ. Microbiol. 61 1599–1609 Occurrence Handle7538280

    PubMed  Google Scholar 

  • J Versalovic M Schneider F J Bruijn Particlede J R Lupski (1994) ArticleTitleGenomic fingerprinting of bacteria using Repetitive sequenced-based Polymerase Chain Reaction Methods Mol. Cell. Biol. 5 25–40 Occurrence Handle1:CAS:528:DyaK2MXhtVOmsLc%3D

    CAS  Google Scholar 

  • J M Vincent (1970) A Manual for the Practical Study of Root Nodule Bacteria. IBP Handbook N° 15 Blackwell Oxford, UK

    Google Scholar 

  • P S Walsh D A Metzger R Higuchi (1991) ArticleTitleChelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material BioTechniques 10 506–513 Occurrence Handle1867860 Occurrence Handle1:STN:280:DyaK3MzivFSjtw%3D%3D

    PubMed  CAS  Google Scholar 

  • W G Weisburg S M Barns D A Pelletier D J Lane (1991) ArticleTitle16S ribosomal DNA amplification for phylogenetic study J. Bacteriol. 173 697–703 Occurrence Handle1987160 Occurrence Handle1:CAS:528:DyaK3MXhsl2lurY%3D

    PubMed  CAS  Google Scholar 

  • Wernegreen J J, Harding E E and Riley M A 1997 Rhizobium gone native: Unexpected plasmid stability of indigenous Rhizobium leguminosarum. In: Proceedings of the National Academy of Sciences of USA. 94, 5483–5488.

  • A Willems M Fernández-López E Muñoz-Adelantado J Goris P Vos ParticleDe E Martínez-Romero N Toro M Gillis (2003) ArticleTitleDescription of new Ensifer strains from nodules and proposal to transfer Ensifer adhaerens Casida 1982 to Sinorhizobium as Sinorhizobium adhaerens comb. nov. Request for an Opinion Int. J. Syst. Evol. Microbiol. 53 1207–1217 Occurrence Handle12892151 Occurrence Handle1:CAS:528:DC%2BD3sXmsF2itLY%3D Occurrence Handle10.1099/ijs.0.02264-0

    Article  PubMed  CAS  Google Scholar 

  • C H Wong R Patchamathu A H Meyer z C E Pankhurst W J Broughton (1988) ArticleTitleRhizobia in tropical legumes: ineffective nodulation of Arachis hypogaea L. by fast growing strains Soil Biol. Biochem. 20 677–681 Occurrence Handle10.1016/0038-0717(88)90152-6

    Article  Google Scholar 

  • J K Yang F L Xie J Zou Q Zhou J C Zhou (2005) ArticleTitlePolyphasic characteristics of bradyrhizobia isolated from nodules of peanut (Arachis hypogaea L.) in China Soil Biol. Biochem. 37 141–153 Occurrence Handle1:CAS:528:DC%2BD2cXovVKntLs%3D Occurrence Handle10.1016/j.soilbio.2004.06.016

    Article  CAS  Google Scholar 

  • J P W Young A W B Johnston (1989) ArticleTitleThe evolution of specificity in the legume-Rhizobium symbiosis Trends. Ecol. Evol. 4 341–349 Occurrence Handle10.1016/0169-5347(89)90089-X

    Article  Google Scholar 

  • X Zhang G Nick S Kaijalainen Z Terefework L Paulin S W Tighe P H Graham K Lindström (1999) ArticleTitlePhylogeny and diversity of Bradyrhizobium isolated from root nodules of peanut (Arachis hypogaea) in Sichuan, China Syst. Appl. Microbiol. 22 378–386 Occurrence Handle10553290 Occurrence Handle1:CAS:528:DyaK1MXmvVensbs%3D

    PubMed  CAS  Google Scholar 

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  1. Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-químicas y Naturales, Universidad Nacional de Río Cuarto, 5800, Río Cuarto, Córdoba, Argentina

    Tania Taurian, Fernando Ibañez & Adriana Fabra

  2. Instituto de Bioquímica y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 1900, La Plata, Argentina

    O. Mario Aguilar

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  1. Tania Taurian
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  2. Fernando Ibañez
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Correspondence to Tania Taurian.

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Taurian, T., Ibañez, F., Fabra, A. et al. Genetic Diversity of Rhizobia Nodulating Arachis hypogaea L. in Central Argentinean Soils. Plant Soil 282, 41–52 (2006). https://doi.org/10.1007/s11104-005-5314-5

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