Abstract
This work studied the effect of two cell-surface lectins isolated from the nitrogen-fixing soil bacterium Azospirillum brasilense Sp7 and from its mutant defective in hemagglutinating activity, A. brasilense Sp7.2.3, on the activities of α-glucosidase, β-glucosidase and β-galactosidase in the exocomponent, membrane and apoplast fractions of wheat-seedling roots. Lectin (40 μg mL−1) incubation for 1 h of the plant fractions increased the enzymes’ activities; both wild-type and mutant lectins were most stimulatory to the activities of all the exocomponent-fraction enzymes studied and to the apoplast-fraction β-glucosidase. Pretreatment of the lectins with their carbohydrate hapten, L-fucose, lowered the effect. The observed differences in the lectins’ ability to influence enzyme catalytic activity are explained by change in the antigenic properties of the mutant lectin.
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Alen’kina, S.A., Payusova, O.A. & Nikitina, V.E. Effect of Azospirillum Lectins on the Activities of Wheat-root Hydrolytic Enzymes. Plant Soil 283, 147–151 (2006). https://doi.org/10.1007/s11104-005-4890-8
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DOI: https://doi.org/10.1007/s11104-005-4890-8