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Sumoylation of Arabidopsis heat shock factor A2 (HsfA2) modifies its activity during acquired thermotholerance

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Abstract

Post-translational modification of target proteins by the small ubiquitin-like modifier protein (SUMO) regulate many cellular processes. In this work we show SUMOylation of the heat shock transcription factor, AtHsfA2, in connection with the plant’s response to heat stress and acquired thermotolerance. Using the Yeast two hybrid and the bimolecular fluorescence complementation system, we have found that AtSUMO1 physically interacts with AtHsfA2. Further investigation allowed us to determine that Lys 315 of AtHsfA2 is the main SUMOylation site. Overexpression of AtSUMO1 led to a decrease in AtHsfA2 transcriptional activation of heat shock promoters. We have examined the effect of AtSUMO1 on AtHsfA2 during heat shock treatments. The phenotype of seedlings overexpressing AtSUMO1 resembled the phenotype of AtHsfA2 knock out seedlings, which were more sensitive than wild type seedlings to repeated heat treatment. Furthermore, AtSUMO1 overexpressing seedlings exhibited lower expression levels of small heat shock proteins as compared with wild type seedlings after heat treatment. Based on our findings, we suggest that AtSUMO1 is involved in the regulation of AtHsfA2 in acquired thermotolerance.

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Abbreviations

HSP:

Heat shock protein

HSF:

Heat shock factor

SUMO:

Small ubiquitin-like modifier protein

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Acknowledgments

This work was partly supported by German–Israeli BioDisc program, by the Israel Science Foundation administered by the Israel Academy of Science and Humanities no. 294/07 and by Research Grant Award no. 3922-06R from BARD, The United States–Israel Binational Agriculture Research and Development Fund. We thank Dr. Pascal von Koskull-Doring for providing the AtHsfA2 knockout plants. We thank Dr. Adam for providing us with antibodies against sHSPs-CI. We would like to thank Ms Maya Bar and Dr. Keren Aviezer-Hagai for helpful suggestions and discussions.

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  1. Department of Plant Sciences, Tel Aviv University, 69978, Tel Aviv, Israel

    Reut Cohen-Peer, Silvia Schuster, David Meiri, Adina Breiman & Adi Avni

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  1. Reut Cohen-Peer
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  2. Silvia Schuster
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Correspondence to Adi Avni.

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Cohen-Peer, R., Schuster, S., Meiri, D. et al. Sumoylation of Arabidopsis heat shock factor A2 (HsfA2) modifies its activity during acquired thermotholerance. Plant Mol Biol 74, 33–45 (2010). https://doi.org/10.1007/s11103-010-9652-1

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