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Dwarf 88, a novel putative esterase gene affecting architecture of rice plant

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Abstract

Rice architecture is an important agronomic trait that affects grain yield. We characterized a tillering dwarf mutant d88 derived from Oryza sativa ssp. japonica cultivar Lansheng treated with EMS. The mutant had excessive shorter tillers and smaller panicles and seeds compared to the wild-type. A reduction in number and size of parenchyma cells around stem marrow cavity as well as a delay in the elongation of parenchyma cells caused slender tillers and dwarfism in the d88 mutant. The D88 gene was isolated via map-based cloning and identified to encode a putative esterase. The gene was expressed in most rice organs, with especially high levels in the vascular tissues. The mutant carried a nucleotide substitution in the first exon of the gene that led to the substitution of arginine for glycine, which presumably disrupted the functionally conserved N-myristoylation domain of the protein. The function of the gene was confirmed by complementation test and antisense analysis. D88, thus, represents a new category of genes that regulates cell growth and organ development and consequently plant architecture. The potential relationship between the tiller formation associated genes and D88 is discussed and future identification of the substrate for D88 may lead to the characterization of new pathways regulating plant development.

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Abbreviations

CAPS:

Cleaved amplified polymorphic sequences

CDAP:

Cell death associated protein

d88 :

dwarf 88

EMS:

Ethyl methane sulfonate

FAA:

Formalin–acetic acid–alcohol

FC1 :

FINE CULM 1

GA:

Gibberellic acid

LRR:

Leucine rich repeat

moc1 :

Mono culm 1

ORFs:

Open reading frames

OsCCD8:

Oryza sativa carotenoid cleavage dioxygenase 8

PCR:

Polymerase chain reaction

PME:

Pectin methyl esterase

RT:

Reverse transcriptase

SD:

Standard deviation

SSR:

Simple sequence repeat

STS:

Sequence tagged site

TB1 :

TEOSINTE BRANCHED 1

References

  • Akiyama K, Matsuzaki K, Hayashi H (2005) Plant sesquiterpenes induce hyphal branching in arbuscular mycorrhizal fungi. Nature 435:824–827

    Article  PubMed  CAS  Google Scholar 

  • Arite T, Iwata H, Ohshima K, Maekawa M, Nakajima M, Kojima M, Sakakibara H, Kyozuka J (2007) DWARF10, an RMS1/MAX4/DAD1 ortholog, controls lateral bud outgrowth in rice. Plant J 51:1019–1029

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Fontana F (2007) Isolation of differentially expressed transcripts–after treatment of platanus acerifolia leaves with cerato-platanin, a multi-functional protein from ceratocystis platani. J Plant Pathol 89(Suppl):S3

    Google Scholar 

  • Fridman E, Wang J, Iijima Y, Froehlich JE, Gang DR, Ohlrogge J, Pichersky E (2005) Metabolic, genomic, and biochemical analyses of glandular trichomes from the wild tomato species Lycopersicon hirsutum identify a key enzyme in the biosynthesis of methylketones. Plant Cell 17:1252–1267

    Article  PubMed  CAS  Google Scholar 

  • Gao Z, Wang P, Hong F, Xie X (1994) Change of esterase isozyme and endogenous hormone in root system and ontogenesis of creeping-rooted character in alfalfa. Acta Agrestia Sinca 2:1–11

    Google Scholar 

  • Gomez-Roldan V, Fermas S, Brewer PB, Puech-Pagès V, Dun EA, Pillot JP, Letisse F, Matusova R, Danoun S, Portais JC, Bouwmeester H, Bécard G, Beveridge CA, Rameau C, Rochange SF (2008) Strigolactone inhibition of shoot branching. Nature 455:189–194

    Article  PubMed  CAS  Google Scholar 

  • Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6:271–282

    Article  PubMed  CAS  Google Scholar 

  • Hubbard L, McSterrn P, Doebley J, Hake S (2002) Expression patterns and mutant phenotype of teosinte branched1 correlate with growth suppression in maize and teosinte. Genetics 162:1927–1935

    PubMed  CAS  Google Scholar 

  • Ishikawa S, Maekawa M, Arite T, Ohnishi K, Takamure I, Kyozuka J (2005) Suppression of tiller bud activity in tillering dwarf mutants of rice. Plant Cell Physiol 46:79–86

    Article  PubMed  CAS  Google Scholar 

  • Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907

    PubMed  CAS  Google Scholar 

  • Kishimoto N, Shimosaka E, Matsuura S, Saito A (1992) A current RFLP linkage map of rice alignment of molecular map with the classical map. Rice Genetics Newslett 9:118–124

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg L (1987) Mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Li X, Qian Q, Fu Z, Wang Y, Xiong G, Zeng D, Wang X, Liu X, Teng S, Hiroshi F, Yuan M, Luok D, Han B, Li Y (2003) Control of tillering in rice. Nature 422:618–621

    Article  PubMed  CAS  Google Scholar 

  • Li H, Xue D, Gao Z, Yan M, Xu W, Xing Z, Huang D, Qian Q, Xue Y (2008) A putative lipase gene EXTRA GLUME1 regulates both empty-glume fate and spikelet development in rice. Plant J 57:593–605

    Article  PubMed  CAS  Google Scholar 

  • Lin H, Wang R, Qian Q, Yan M, Meng X, Fu Z, Yan C, Jiang B, Su Z, Li J, Wang Y (2009) DWARF27, an iron-containing protein required for the biosynthesis of strigolactones, regulates rice tiller bud outgrowth. Plant Cell

  • Matsuoka M, Ichikawa H, Saito A, Tada Y, Fujimura T, Kano-Murakami Y (1993) Expression of a rice homeobox gene causes altered morphology of transgenic plants. Plant Cell 5:1039–1048

    Article  PubMed  CAS  Google Scholar 

  • Matusova R, Rani K, Verstappen FW, Franssen MC, Beale MH, Bouwmeester HJ (2005) The strigolactone germination stimulants of the plant-parasitic Striga and Orobanche spp. are derived from the carotenoid pathway. Plant Physiol 139:920–934

    Article  PubMed  CAS  Google Scholar 

  • Page RDM (1996) TREEVIEW: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    PubMed  CAS  Google Scholar 

  • Qian Q, Li YH, Zeng D, Teng S, Wang Z, Li X, Dong Z, Dai N, Sun L, Li J (2001) Isolation and genetic characterization of a fragile plant mutant rice (Oryza sativa L.). Chin Sci Bull 46:2082–2085

    Article  CAS  Google Scholar 

  • Reintanz B, Lehnen M, Reichelt M, Gershenzon J, Kowalczyk M, Sandberg G, GoddeM UhlR, Palme K (2001) Bus, a bushy Arabidopsis CYP79F1 knockout mutant with abolished synthesis of short-chain aliphatic glucosinolates. Plant Cell 13:351–367

    Article  PubMed  CAS  Google Scholar 

  • Ren CW, Kermode AR (2000) An increase in pectin methyl esterase activity accompanies dormancy breakage and germination of yellow cedar seeds. Plant Physiol 124:231–242

    Article  PubMed  CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Takeda K (1977) Internode elongation and dwarfism in some gramineous plants. Gamma Field Sym 16:1–18

    Google Scholar 

  • Takeda T, Suwa Y, Kitano H, Ueguchi-Tanaka M, Ashikari M, Matsuoka M, Ueguchi C (2003) The OsTB1 gene negatively regulates lateral branching in rice. Plant J 33:513–520

    Article  PubMed  CAS  Google Scholar 

  • Ueguchi-Tanaka M, Ashikari M, Nakajima M, Itoh H, Katoh E, Kobayashi M, Chow TY, Hsing YI, Kitano H, Yamaguchi I, Matsuoka M (2005) GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin. Nature 437:693–698

    Article  PubMed  CAS  Google Scholar 

  • Umehara M, Hanada A, Yoshida S, Akiyama K, Arite T, Takeda-Kamiya N, Magome H, Kamiya Y, Shirasu K, Yoneyama K, Kyozuka J, Yamaguchi S (2008) Inhibition of shoot branching by new terpenoid plant hormones. Nature 455:195–200

    Article  PubMed  CAS  Google Scholar 

  • Wadsworth GJ, Redinbaugh MG, Scandalios JG (1988) A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis. Analyt Biochem 172:279–283

    Article  PubMed  CAS  Google Scholar 

  • Wang Y, Li J (2008) Molecular basis of plant architecture. Annu Rev Plant Biol 59:253–279

    Article  PubMed  CAS  Google Scholar 

  • Ward SP, Leyser O (2004) Shoot branching. Curr Opin Plant Biol 7:73–78

    Article  PubMed  CAS  Google Scholar 

  • Wäspie U, Misteli B, Hasslacher M, Jandrositz A, Kohlwein SD, Schwab H, Dudler R (1998) The defense-related rice gene Pir7b encodes an α/β hydrolase fold protein exhibiting esterase activity towards naphthol AS-esters. Eur J Biochem 254:32–37

    Article  Google Scholar 

  • Yan H, Saika H, Maekawa M, Takamure I, Tsutsumi N, Kyozuka J, Nakazono M (2007) Rice tillering dwarf mutant dwarf3 has increased leaf longevity during darkness-induced senescence or hydrogen peroxide-induced cell death. Genes Genet Syst 82:361–366

    Article  PubMed  CAS  Google Scholar 

  • Zhong G, Goren R, Riov J, Sisler EC, Holland D (2001) Characterization of an ethylene-induced esterase gene isolated from Citrus sinensis by competitive hybridization. Physiol Plantarum 113:267–274

    Article  CAS  Google Scholar 

  • Zou J, Chen Z, Zhang S, Zhang W, Jiang G, Zhao X, Zhai W, Pan X, Zhu L (2005) Characterizations and fine mapping of a mutant gene for high tillering and dwarf in rice (Oryza sativa L.). Planta 222:604–612

    Article  PubMed  CAS  Google Scholar 

  • Zou J, Zhang S, Zhang W, Li G, Chen Z, Zhai W, Zhao X, Pan X, Xie Q, Zhu L (2006) The rice HIGH-TILLERING DWARF1 encoding an ortholog of Arabidopsis MAX3 is required for negative regulation of the outgrowth of axillary buds. Plant J 48:687–696

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from the Ministry of Science and Technology of China (2006AA10A102), the Chinese Academy of Sciences (KSCW2-YW-N-024), and the National Natural Science Foundation of China (Grant Nos. 30400255 and 30821004). We thank Prof. Gao Xiaoyan at Institute of Plant Physiology and Ecology in Shanghai for histological analysis of stem base sections of Lansheng and d88. We are grateful to Ms. Yan Honglan at China National Rice Research Institute for photograph, Dr. Huang Xuehui at National Center for Gene Research for critical reading of the manuscript.

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Authors and Affiliations

  1. National Center for Gene Research/Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, 200233, Shanghai, China

    Zhenyu Gao, Xiaohui Liu, Qi Feng & Bin Han

  2. State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, 310006, Hangzhou, China

    Zhenyu Gao, Qian Qian, Meixian Yan, Guojun Dong & Jian Liu

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  1. Zhenyu Gao
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  2. Qian Qian
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  3. Xiaohui Liu
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Correspondence to Qian Qian or Bin Han.

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Gao, Z., Qian, Q., Liu, X. et al. Dwarf 88, a novel putative esterase gene affecting architecture of rice plant. Plant Mol Biol 71, 265–276 (2009). https://doi.org/10.1007/s11103-009-9522-x

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