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Retrograde regulation of nuclear gene expression in CW-CMS of rice

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Abstract

The CW-cytoplasmic male sterility (CMS) line has the cytoplasm of Oryza rufipogon Griff, and mature pollen is morphologically normal under an optical microscope but lacks the ability to germinate; restorer gene Rf17 has been identified as restoring this ability. The difference between nuclear gene expression in mature anthers was compared for the CW-CMS line, [cms-CW] rf17rf17, and a maintainer line with normal cytoplasm of Oryza sativa L., [normal] rf17rf17. Using a 22-k rice oligoarray we detected 58 genes that were up-regulated more than threefold in the CW-CMS line. Expression in other organs was further investigated for 20 genes using RT-PCR. Five genes, including genes for alternative oxidase, were found to be preferentially expressed in [cms-CW] rf17rf17 but not in [normal] rf17rf17 or [cms-CW] Rf17Rf17. Such [cms-CW] rf17rf17-specific gene expression was only observed in mature anthers but not in leaves, stems, or roots, indicating the presence of anther-specific mitochondrial retrograde regulation of nuclear gene expression, and that Rf17 has a role in restoring the ectopic gene expression. We also used a proteomic approach to discover the retrograde regulated proteins and identified six proteins that were accumulated differently. These results reveal organ-specific induced mitochondrial retrograde pathways affecting nuclear gene expression possibly related to CMS.

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Abbreviations

CMS:

cytoplasmic male sterility

Rf :

fertility restorer gene

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Acknowledgements

This study was partially supported by a grant from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Green Technology Project QT-2007), and by a Grant-in-Aid for Special Research on Priority Areas (No. 18075002) from the Ministry of Education, Science, Sports, and Culture, Japan. S.F. is the recipient of Research Fellowships of the Japan Society for the promotion of Science for Young Scientists. We thank the Rice Genome Resource Center at the National Institute of Agrobiological Sciences, Japan, for use of the rice microarray analysis system and the technical support provided by Dr Nagamura and Mrs Motoyama.

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Authors and Affiliations

  1. Laboratory of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555, Japan

    Sota Fujii & Kinya Toriyama

  2. Laboratory of Gene Regulation, Department of Molecular Genetics, National Institute of Agrobiological Sciences, Tsukuba, 305-8602, Japan

    Setsuko Komatsu

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  1. Sota Fujii
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  2. Setsuko Komatsu
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Correspondence to Kinya Toriyama.

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Fujii, S., Komatsu, S. & Toriyama, K. Retrograde regulation of nuclear gene expression in CW-CMS of rice. Plant Mol Biol 63, 405–417 (2007). https://doi.org/10.1007/s11103-006-9097-8

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