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Antisense suppression of thioredoxinhmRNA in Brassica napus cv.

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Abstract

In Brassica, the thioredoxinhproteins, THL1 and THL2, were previously found to be potential inhibitors of the S receptor kinase (SRK) in the Brassica self-incompatibilty response. To investigate the biological roles of THL1 and THL2 in pollen–pistil interactions, the stigma-specific SLR1 promoter was used to drive antisense THL1/2 expression in Brassica napus cv. Westar. This cultivar is normally compatible, but antisense suppression of THL1/2 led to a low level constitutive rejection of all Brassica napus pollen tested. Fluorescence microscopy revealed that the pollen rejection was a typical Brassica self-incompatibility rejection response with reduced pollen adhesion, germination and pollen tube growth. In addition, Westar was found to express the SLG15 and SRK15 proteins which may be the target of regulation by THL1 and THL2. Thus, these results indicate that the THL1 and THL2 are required for full pollen acceptance in B. napus cv. Westar.

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Authors and Affiliations

  1. Department of Botany, University of Toronto, Ontario, Canada

    Yosr Haffani & Daphne Goring

  2. Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Ontario, Canada

    Yosr Haffani

  3. Reproduction et Développement des Plantes, UMR 5667 CNRS-INRA-ENSL-, Université de Lyon, IFR 128 BioSciences Lyon-Gerland, École Normale Supérieure de Lyon, 46, allée d'Italie, France

    Thierry Gaude & J. Cock

  4. Unité Mixte de Recherche 7139, CNRS-Goemar-Université, Paris 6, Station Biologique, Place Georges Teissier, BP 74, France

    J. Cock

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  1. Yosr Haffani
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  2. Thierry Gaude
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  3. J. Cock
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Haffani, Y., Gaude, T., Cock, J. et al. Antisense suppression of thioredoxinhmRNA in Brassica napus cv. . Plant Mol Biol 55, 619–630 (2004). https://doi.org/10.1007/s11103-004-1126-x

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