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Ag – ZnO Nanocomposites Cause Cytotoxicity and Induce Cell Cycle Arrest in Human Gastric and Melanoma Cancer Cells

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Pharmaceutical Chemistry Journal Aims and scope

Zinc oxide nanoparticles are used increasingly as antimicrobial and therapeutic agents, and the addition of metal ions such as silver may improve their potent cytotoxicity. However, there have been concerns about safety. In this work, we investigated the cytotoxic activity of newly synthesized silver/zinc oxide nanocomposites (Ag-ZnO NCs) versus ZnO nanoparticles (NPs) against human melanoma (A375) and gastric carcinoma (AGS). The cytotoxicity of Ag-ZnO NCs versus ZnO NPs was evaluated by cell viability assays and the cell cycle analyses were performed by flow cytometry using DAPI staining. Both ZnO NPs and Ag-ZnO NCs significantly reduced cell viability in a dose-dependent manner. We found that Ag-ZnO NCs cytotoxicity was lower than that of ZnO NPs in the same concentration range. Furthermore, the cytotoxicity caused by Ag-ZnO NCs and ZnO induced the accumulation of melanoma cells in S phase and gastric cancer cells in G2/M phase. It was concluded that Ag-ZnO NCs were less toxic than ZnO NPs. This approach provides a rational basis for evaluating the potential harm of ZnO NPs and Ag-ZnO NCs as food packaging materials and cancer therapy agents.

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Acknowledgments

This work was supported by a grant from the Ardabil University of Medical Sciences, Grant No 89350.

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Authors and Affiliations

  1. Department of Biology, Payame Noor University, Tehran, 5618985991, Iran

    Mina Mahdavi Rad

  2. Research Laboratory for Embryology and Stem Cells, Department of Anatomy and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, 5618985991, Iran

    Nowruz Najafzadeh

  3. Department of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, 5618985991, Iran

    Nasrin Tata

  4. Antimicrobial Resistance Research Center, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, 5618985991, Iran

    Alireza Jafari

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  1. Mina Mahdavi Rad
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  2. Nowruz Najafzadeh
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Contributions

N. N. supervised and coordinated the study and finalized the manuscript. The study design, data analyses, and manuscript writing were performed by N. N, M. M, N. T, and A. J. All authors have given approval to the final version of the manuscript.

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Correspondence to Nowruz Najafzadeh.

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Rad, M.M., Najafzadeh, N., Tata, N. et al. Ag – ZnO Nanocomposites Cause Cytotoxicity and Induce Cell Cycle Arrest in Human Gastric and Melanoma Cancer Cells. Pharm Chem J 52, 112–116 (2018). https://doi.org/10.1007/s11094-018-1774-9

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  • DOI: https://doi.org/10.1007/s11094-018-1774-9

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