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Quercetin-loaded PLGA nanoparticles: a highly effective antibacterial agent in vitro and anti-infection application in vivo

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Abstract

Nanotechnology-based approaches have tremendous potential for enhancing efficacy against infectious diseases. PLGA-based nanoparticles as drug delivery carrier have shown promising potential, owing to their sizes and related unique properties. This article aims to develop nanosized poly (d, l-lactide-co-glycolide) PLGA nanoparticle formulation loaded with quercetin (QT). QT is an antioxidant and antibacterial compound isolated from Chinese traditional medicine with low skin permeability and extreme water insolubility. The quercetin-loaded PLGA nanoparticles (PQTs) were synthesized by emulsion–solvent evaporation method and stabilized by coating with poly (vinyl alcohol). The characteristics of PQTs were analyzed by Fourier transform infrared spectroscopy, Ultraviolet–Visible spectroscopy, scanning electron microscope, transmission electron microscopy, and atomic force microscopy, respectively. The PQTs showed a spherical shape with an average size of 100–150 nm. We compared the antibacterial effects of PQTs against Escherichia coli (E. coli) and Micrococcus tetragenus (M. tetragenus).The PQTs produced stronger antibacterial activity to E. coli than that to M. tetragenus through disrupting bacterial cell wall integrity. The antibacterial ratio was increased with the increasing dosages and incubation time. Next, we tested the in vivo antibacterial activity in mice. No noticeable organ damage was captured from H&E-staining organ slices, suggesting the promise of using PQTs for in vivo applications. The results of this study demonstrated the interaction between bacteria and PLGA-based nanoparticles, providing encouragement for conducting further investigations on properties and antimicrobial activity of the PQTs in clinical application.

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Acknowledgments

The authors gratefully acknowledge the financial supports from the National Natural Science Foundation of China (21401002); the Natural Science Foundation of Anhui Province, China (1508085QB37); the Youth Science Found Key Project of Anhui Agricultural University (2013zr011); the Science Foundation of Young Teachers of Anhui Agricultural University (ZHSJ2013057); and the New Specialty of Biopharmaceutical Construction of Anhui Agricultural University (SJJD201313).

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    Authors and Affiliations

    1. School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China

      Dongdong Sun, Nuan Li, Weiwei Zhang, Endong Yang, Zhipeng Mou, Zhiwei Zhao, Haiping Liu & Weiyun Wang

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    1. Dongdong Sun
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    2. Nuan Li
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    3. Weiwei Zhang
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    8. Weiyun Wang
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    Correspondence to Weiyun Wang.

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    Dongdong Sun and Nuan Li have contributed equally to this work.

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    Sun, D., Li, N., Zhang, W. et al. Quercetin-loaded PLGA nanoparticles: a highly effective antibacterial agent in vitro and anti-infection application in vivo. J Nanopart Res 18, 3 (2016). https://doi.org/10.1007/s11051-015-3310-0

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