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Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity

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Abstract

Development of green nanotechnology is generating interest of researchers toward ecofriendly biosynthesis of nanoparticles. In this study, biosynthesis of stable silver nanoparticles was done using Tulsi (Ocimum sanctum) leaf extract. These biosynthesized nanoparticles were characterized with the help of UV–vis spectrophotometer, Atomic Absorption Spectroscopy (AAS), Dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Transmission electron microscopy (TEM). Stability of bioreduced silver nanoparticles was analyzed using UV–vis absorption spectra, and their antimicrobial activity was screened against both gram-negative and gram-positive microorganisms. It was observed that O. sanctum leaf extract can reduce silver ions into silver nanoparticles within 8 min of reaction time. Thus, this method can be used for rapid and ecofriendly biosynthesis of stable silver nanoparticles of size range 4–30 nm possessing antimicrobial activity suggesting their possible application in medical industry.

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References

  • Ahmad A, Senapati S, Khan IM, Kumar R, Sastry M (2003a) Extracellular biosynthesis of monodisperse gold nanoparticles by a novel extremophilic actinomycete Thermomonospora sp. Langmuir 19:3550–3553

    Article  CAS  Google Scholar 

  • Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan MI, Kumar R et al (2003b) Extracellular biosynthesis of silver nanoparticles using fungus Fusarium oxysporium. Colloids Surf B Biointerfaces 28:313–318

    Article  CAS  Google Scholar 

  • Arangasamy L, Munusamy V (2008) Tapping the unexploited plant resources for the synthesis of silver nanoparticles. Afr J Biotechnol 7(17):3162–3165

    CAS  Google Scholar 

  • Becker RO (1999) Silver ions in the treatment of local infections. Met Based Drugs 6:297–300

    Article  Google Scholar 

  • Catauro M, Raucci MG, De Gaaetano FD, Marotta A (2005) Sol–gel processing of drug delivery materials and release kinetics. J Mater Sci Mater Med 16(3):261–265

    Article  Google Scholar 

  • Chandran SP, Chaudhary M, Pasricha R, Ahmad A, Sastry M (2000) Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract. Biotechnol Prog 22:577–583

    Article  Google Scholar 

  • Crabtree JH, Brruchette RJ, Siddiqi Ra, Huen IT, Handott LL, Fishman A (2003) The efficacy of silver-ion implanted catheters in reducing peritoneal dialysis-related infections. Perit Dial Int 23(4):368–374

    CAS  Google Scholar 

  • Dickson DPE (1999) Nanostructured magnetism in living systems. J Magn Magn Mater 203:46–49

    Article  CAS  Google Scholar 

  • Duran N, Marcato PD, Alves OL, De Souza GIH, Esposito E (2005) Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. J Nanobiotechnol 3:8–14

    Article  Google Scholar 

  • Eftink MK, Ghiron CA (1981) Fluorescence quenching studies with proteins. Anal Biochem 114:199–227

    Article  CAS  Google Scholar 

  • Fayaz AM, Balaji K, Girilal M, Yadav R, Kalaichelvan PT, Venketesan R (2010) Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria. Nanomed Nanotechnol Biol Med 6:103–109

    Article  CAS  Google Scholar 

  • Huang J, Li Q, Sun D, Lu Y, Su Y, Yang X, Wang H, Wang Y, Shao W, He N, Hong J, Chen C (2007) Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf. Nanotechnology 18:105104–105114

    Article  Google Scholar 

  • Huang NM, Lim HN, Radiman S, Khiew PS, Chiu WS, Hashin R, Chia CH (2010) Sucrose ester micellar-mediated synthesis of Ag nanoparticles and their antibacterial properties. Colloids Surf 353:69–76

    Article  CAS  Google Scholar 

  • Jiang H, Manolache S, Wong ACL, Denes FS (2004) Plasma enhanced deposition of silver nanoparticles onto polymer and metal surfaces for the generation of antimicrobial characteristics. J Appl Polym Sci 93:1411–1422

    Article  CAS  Google Scholar 

  • Jilie K, Shaoning YU (2007) Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta Biochim Biophys Sin 39(8):549–559

    Article  Google Scholar 

  • Joerger R, Klaus T, Granqvist CG (2001) Bio-logically produced silver–carbon composite materials for optically functional thin-film coating. Adv Mater 12:407–409

    Article  Google Scholar 

  • Klaus T, Joerger R, Olsson E, Granqvist C-G (1999) Silver-based crystalline nanoparticles, microbially fabricated. Proc Natl Acad Sci USA 96:13611–13614

    Article  CAS  Google Scholar 

  • Klug HP, Alexander LE (1967) X-ray diffraction procedures for polycrystalline and amorphous materials. Wiley, New York

    Google Scholar 

  • Krolikowska A, Kudelski A, Michota A, Bukowska J (2003) SERS studies on the structure of thioglycolic acid monolayers on silver and gold. Surf Sci 532:227–232

    Article  Google Scholar 

  • Li S, Shen Y, Xie A, Yu X, Qiu L, Zhang L, Zhang O (2007) Green synthesis of silver nanoparticles using Capsicum annuum L. extract. Green Chem 9:852–858

    Article  CAS  Google Scholar 

  • Macdonald IDG, Smith WE (1996) Orientation of cytochrome C adsorbed on a citrate-reduced silver colloid surface. Langmuir 12:706–713

    Article  CAS  Google Scholar 

  • Mayr-Harting A, Hedges A, Berkeley R, eds (1972) Methods for studying bactericides. Academic Press, New York, p 74

  • Mukherjee P, Ahmad A, Mandal DS, Senapati S, Sainkar R, Khan MI, Parishcha R, Ajaykumar PV, Alam M, Kumar R, Sastry M (2001) Fungus-mediated synthesis of silver nanoparticles and their immobilization in the mycelial matrix: a novel biological approach to nanoparticle synthesis. Nano Lett 1:515–519

    Article  CAS  Google Scholar 

  • Mulvaney P (1996) Surface plasmon spectroscopy of nanosized metal particles. Langmuir 12:788–800

    Article  CAS  Google Scholar 

  • Nair B, Pradeep T (2002) Coalescence of nanoclusters and the formation of sub-micron crystallites assisted by Lactobacillus strains. Cryst Growth Des 2:293–298

    Article  CAS  Google Scholar 

  • Pum D, Sleytr UB (1999) The application of bacterial S-layers in molecular nanotechnology. Trends Biotechnol 17:8–12

    Article  CAS  Google Scholar 

  • Sathyavathi R, Krishna MB, Rao SV, Saritha R, Rao DN (2010) Biosynthesis of silver nanoparticles using Coriandrum Sativum leaf extract and their application in nonlinear optics. Adv Sci Lett 3:1–6

    Google Scholar 

  • Shankar SS, Ahmad A, Pasricha R, Sastry M (2003) Bioreduction of chloroaurate ions by geranium leaves and its endophytic fungus yields gold nanoparticles of different shapes. J Mater Chem 13:1822–1826

    Article  CAS  Google Scholar 

  • Shankar SS, Rai A, Ahmad A, Sastry M (2004) Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. J Colloid Interface Sci 275:496–502

    Article  CAS  Google Scholar 

  • Shankar SS, Rai A, Ahmad A, Sastry M (2005) Controlling the optical properties of lemongrass extract synthesized gold nanotriangles and potential application in infrared-absorbing optical coatings. Chem Mater 17:566–572

    Article  CAS  Google Scholar 

  • Shrivastava S, Bera T, Roy A, Singh G, Ramachandrarao P, Dash D (2007) Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology 18:225103

    Article  Google Scholar 

  • Silver S (2003) Bacterial silver resistance: molecular biology and uses and misuses of silver compounds. FEMS Microbiol Rev 27:341–353

    Article  CAS  Google Scholar 

  • Sondi I, Goia DV, Matijevi′c E (2003) Preparation of highly concentrated stable dispersions of uniform silver nanoparticles. J Colloid Interface Sci 260:75–81

    Article  CAS  Google Scholar 

  • Song JY, Kim BS (2009) Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst Eng 32:79–84

    Article  Google Scholar 

  • Spring H, Schleifer KH (1995) Diversity of magnetotactic bacteria. Syst Appl Microbiol 18(2):147–153

    Google Scholar 

  • Zhao G, Stevens JS (1998) Multiple parameters for the comprehensive evaluation of the susceptibility of Escherichia coli to the silver ion. Biometals 11:27

    Article  CAS  Google Scholar 

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Acknowledgments

We heartly acknowledge Dr. A. K. Chauhan for their support and providing facilities for the fulfillment of this study. We also acknowledge Dr. Richa Krishna for her guidance.

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Authors and Affiliations

  1. Amity Institute of Nanotechnology, Amity University Uttar Pradesh, Noida, Uttar Pradesh, India

    Garima Singhal, Riju Bhavesh, Kunal Kasariya & Rajendra Pal Singh

  2. Infectious Disease Medical Research Center, College of Medicine, Hallym University, Chuncheon, Gangwan Do, South Korea

    Ashish Ranjan Sharma

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  1. Garima Singhal
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  2. Riju Bhavesh
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  3. Kunal Kasariya
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  4. Ashish Ranjan Sharma
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  5. Rajendra Pal Singh
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Correspondence to Garima Singhal.

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Singhal, G., Bhavesh, R., Kasariya, K. et al. Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. J Nanopart Res 13, 2981–2988 (2011). https://doi.org/10.1007/s11051-010-0193-y

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  • DOI: https://doi.org/10.1007/s11051-010-0193-y

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