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Comparison of Water, Oils and Emulsifiable Adjuvant Oils as Formulating Agents for Metarhizium Anisopliae for Use in Control of Boophilus Microplus

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Abstract

Studies were conducted to identify oil-based formulating agents (paraffinic oil, palm oil and emulsifiable adjuvant oils (EAOs)) for Metarhizium anisopliae that were superior to water with simple surfactants using a germination test and a bioassay against Boophilus microplus. Germination of conidia in all formulations, except 10% coconut EAO, produced more than 68% germination at 24 h and nearly 100% at 48 h. Coconut oil (average survival time (AST)=4.6±0.28 days) and 10% liquid paraffin EAO (AST=4.4±0.15 days) enhanced the pathogenicity of M. anisopliae to B. microplus relative to water (AST=8.4±0.42 days). M. anisopliae in 10% liquid paraffin EAO was the most effective formulation having a moderately high germination after 24 h and a low AST as well as a high AST in the control. In the second experiment, germination of conidia in 2% liquid paraffin EAO and 2% Cropspray was higher than in 2% Codacide oil at 24 h, however, all treatments reached 100% germination after 48 h. The ASTs of the EAO based M. anisopliae formulations (Average AST=6.4±0.54 days) were similar but lower that the ASTs of the controls (Average AST=9.6±0.28 days).

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Authors and Affiliations

  1. CAB International Caribbean and Latin America Regional Centre, Curepe, Trinidad and Tobago

    Perry Polar & Sally-Ann John

  2. Center for Biological Control, College of Engineering Sciences, Technology and Agriculture, Texas A&M University, Florida, USA

    Moses T.K. Kairo

  3. CAB International UK Centre, Bakeham Lane, TW20 9TY, Egham, Surrey, UK

    Dave Moore

  4. FAO-Caribbean Amblyomma Programme, Friars Hill Road, P.O. Box W 1572, St Johns, Antigua

    Rupert Pegram

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  1. Perry Polar
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  2. Moses T.K. Kairo
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  3. Dave Moore
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  4. Rupert Pegram
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  5. Sally-Ann John
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Correspondence to Perry Polar.

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Polar, P., Kairo, M.T., Moore, D. et al. Comparison of Water, Oils and Emulsifiable Adjuvant Oils as Formulating Agents for Metarhizium Anisopliae for Use in Control of Boophilus Microplus. Mycopathologia 160, 151–157 (2005). https://doi.org/10.1007/s11046-005-0120-4

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  • DOI: https://doi.org/10.1007/s11046-005-0120-4

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