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Genetic structure and diversity analysis of tall fescue populations by EST-SSR and ISSR markers

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Abstract

Tall fescue is a perennial cool-season grass with economic importance especially in temperate regions of the northern hemisphere. This study was done to assess the genetic diversity and population structure of 90 tall fescue populations and cultivars using ISSR and EST-SSR markers in order to categorize valuable populations for breeding programs and to construct the core collection of tall fescue collection in Iran. The 10 EST-SSR primer pairs amplified 92 alleles. The allele numbers varied from 4 to 13 alleles per locus with an average of 9.2 alleles, of which 84 (90.6%) were polymorphic with an average of 8.4 polymorphic bands per primer. The 39 ISSR primers totally produced 387 scorable bands, of which 335 (86.6%) were polymorphic with an average of 8.6 polymorphic bands per primer. The amplified markers by ISSR primers varied from 6 to 14 markers per primer with an average of 9.92 markers per primer. The 90 tall fescue populations using both EST-SSR and ISSR data were classified into two clusters by UPGMA method that was coincide with PCA and structure analysis results. The turf-type and forage-type populations were clearly separated. Based on the results, the Iranian populations provide a valuable and novel germplasm to employ in tall fescue varietal improvement programs for both forage and turf-type applications. This progression is an important step to introduce this collection for development of a core collection of tall fescue germplasm in Iran.

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Abbreviations

ISSR:

Inter-simple sequence repeat

PCR:

Polymerase chain reaction

EST-SSR:

Expressed sequence tag-simple sequence repeat

UPGMA:

Unweighted pair group method with arithmetic mean

PCA:

Principal component analysis

PIC:

Polymorphic information content

He:

Expected heterozygosity

Ne:

Number of effective alleles

I:

Shannon’s information index

PPL:

Percentage of polymorphic loci

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Acknowledgements

The financial support of the Agricultural Biotechnology Research Institute of Iran (ABRII) is gratefully acknowledged.

Funding

This work was supported by the Agricultural Biotechnology Research Institute of Iran (ABRII) (Grant Numbers 12-05-05-015-96024-960689).

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Authors and Affiliations

  1. Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran

    Z. Shahabzadeh, R. Darvishzadeh & M. Jaffari

  2. Branch for Northwest & West Region, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Tabriz, Iran

    R. Mohammadi

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  1. Z. Shahabzadeh
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Correspondence to R. Mohammadi.

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Shahabzadeh, Z., Mohammadi, R., Darvishzadeh, R. et al. Genetic structure and diversity analysis of tall fescue populations by EST-SSR and ISSR markers. Mol Biol Rep 47, 655–669 (2020). https://doi.org/10.1007/s11033-019-05173-z

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