Abstract
The present study details on the mechanism of synthesis of bis (4-formyl-2 methoxy phenyl carbonate), using two green reagents dimethyl carbonate and vanillin for application as therapeutic agent. The synthesized FMPC was identified from the 13C nuclear magnetic resonance spectra. The novel modified Schiff base nanoparticles resulted from the crosslinking of FMPC with chitosan were confirmed by cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance spectroscopy. The incorporation of the FMPC was identified from the amorphous X-ray diffraction patterns of C-FMPC-Nps. The thermal stability of the formed nanoparticles was predicted using thermogravimetric analysis. The morphology of the nanoparticles as observed from HRTEM was found to be smooth and spherical in nature. Both FMPC and C-FMPC-Nps showed significant radical scavenging potential and anticancer property. The carbonate ester backbone and the moiety present in chitosan-FMPC-nanoparticles, underwent hydrolysis at the targeted cancer causing microenvironment to release vanillin and chitosan and enhance the anticancer activity. Both FMPC and C-FMPC-Nps exhibits a dose dependent cytotoxicity towards the different cell lines and it was tested with a commercial drug for application studies.
Graphical abstract
Effective synthesis of FMPC, successful incorporation onto chitosan nanoparticles for the formation of C-FMPC-Nps. The formed Schiff base compound proves to have enhanced antioxidant and anticancer efficacy.
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Abbreviations
- FMPC:
-
Bis (4-formyl-2 methoxy phenyl carbonate)
- DMC:
-
Dimethyl carbonate
- 13C-NMR:
-
13C nuclear magnetic resonance
- Nps:
-
Nanoparticles
- C:
-
Chitosan
- CP/MAS:
-
13C-NMR cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance
- XRD:
-
X-ray diffraction
- TGA:
-
Thermogravimetric analysis
- HRTEM:
-
High resolution transmission electron microscopy
- TPP:
-
Sodium tripolyphosphate
- ATR:
-
Attenuated total reflectance
- D2O:
-
Deuterium oxide
- EI-MS:
-
Electron ionization mass spectrometry
- ZrO:
-
Zirconium oxide
- KHZ:
-
Kilohertz
- TMS:
-
Tetramethyl silane
- TEA:
-
Triethyl amine
- MTT:
-
[3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]
- DPPH:
-
(1,1-Diphenyl-2-picryl hydrazyl)
- MEM:
-
Minimal essential medium
- FBS:
-
Fetal bovine serum
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Thyriyalakshmi, P., Radha, K.V. Fabrication of chitosan-bis (4-formyl-2 methoxy phenyl carbonate) Schiff base nanoparticles and evaluation of their antioxidant and anticancer properties. Mol Biol Rep 46, 4333–4347 (2019). https://doi.org/10.1007/s11033-019-04887-4
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DOI: https://doi.org/10.1007/s11033-019-04887-4