Abstract
A cDNA encoding translationally controlled tumor protein (TCTP) of Jatropha curcas L., JcTCTP, was isolated from an endosperm cDNA library. JcTCTP consisted of a 5′ untranslated region (UTR) of 526 bp, a 3′ UTR of 377 bp and an open reading frame (ORF) of 507 bp, encoding a protein of 168 amino acid residues, which contained two signature sequences of TCTP family. Its deduced amino acid sequence was similar to the other known plants TCTPs in a range of 77.4–92.3%. Expression of JcTCTP was the highest in the stem, endosperm at embryo formation stage and embryo of J. curcas tissues, and the lowest in the endosperm at seminal leaf embryo stage and flower, demonstrating a pattern of temporal and spatial specific expression.
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Acknowledgments
This research is supported by “11th Five-Year Plan” to support science and technology project (NO.2006BAD07A04, NO.2007BAD50B05), Ministry of Science and Technology international cooperation project (NO.2006DFB63400), National Natural Science Foundation project on the surface (NO.30670204), and the Youth Science and Technology Fund of Sichuan Province (No. 07ZQ026-039).
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Qin, X., Gao, F., Zhang, J. et al. Molecular cloning, characterization and expression of cDNA encoding translationally controlled tumor protein (TCTP) from Jatropha curcas L. Mol Biol Rep 38, 3107–3112 (2011). https://doi.org/10.1007/s11033-010-9980-x
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DOI: https://doi.org/10.1007/s11033-010-9980-x