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Overexpression of CsNMAPK in tobacco enhanced seed germination under salt and osmotic stresses

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Abstract

In this research, biological function of CsNMAPK, encoding a mitogen-activated protein kinase of cucumber, was investigated under salt and osmotic stresses. Northern blot analysis showed that the expression of CsNMAPK was induced by salt and osmotic stresses in the cucumber root. In order to determine whether CsNMAPK was involved in plant tolerance to salt and osmotic stresses, transgenic tobacco plants constitutively overexpressing CsNMAPK were generated. Northern and Western blot analysis showed that strong signals were detected in the RNA and protein samples extracted from transgenic lines, whereas no signal was detected in the wild type tobacco, indicating that CsNMAPK was successfully transferred into tobacco genome and overexpressed. The results of seed germination showed that germination rates of transgenic lines were significantly higher than wild type under high salt and osmotic stresses. In addition, seed growth of transgenic lines was much better than wild type under salt and osmotic stresses. These results indicated that overexpression of CsNMAPK positively regulated plant tolerance to salt and osmotic stresses.

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Acknowledgments

This study has been supported by the National Natural Science Foundation of China (No. 30471187).

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, 271018, People’s Republic of China

    Huini Xu, Fengjuan Yang, Qinghua Shi & Xiufeng Wang

  2. Biotechnology Research Center, Kunming University of Science and Technology, Kunming, 650224, People’s Republic of China

    Huini Xu & Kunzhi Li

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  1. Huini Xu
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  2. Kunzhi Li
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  3. Fengjuan Yang
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  4. Qinghua Shi
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  5. Xiufeng Wang
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Correspondence to Xiufeng Wang.

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Xu, H., Li, K., Yang, F. et al. Overexpression of CsNMAPK in tobacco enhanced seed germination under salt and osmotic stresses. Mol Biol Rep 37, 3157–3163 (2010). https://doi.org/10.1007/s11033-009-9895-6

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  • DOI: https://doi.org/10.1007/s11033-009-9895-6

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