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Fine mapping of Rscmv2, a major gene for resistance to sugarcane mosaic virus in maize

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Abstract

Sugarcane mosaic virus (SCMV) is one of the most devastating virus diseases in maize. In our previous study, two dominant complementary genes, Rscmv1 and Rscmv2, were identified in the resistant inbred line Siyi to confer resistance against SCMV. In this study, we report on the fine mapping of Rscmv2 and prediction of candidate genes. We developed a near-isogenic mapping population comprising 9,856 individuals segregating at Rscmv2, but not the Rscmv1 region. Combining the screening of the recombinants in the Rscmv2 region with 16 newly developed molecular markers, we fine-mapped Rscmv2 to an interval of 196.5 kb. Two overlapping bacterial artificial chromosomes covering this region were analyzed to identify putative candidate genes. Two predicted genes, encoding an auxin-binding protein and a Rho GTPase-activating protein, respectively, can be suggested as candidate genes for Rscmv2. The information from fine-mapping Rscmv2 can contribute to map-based cloning of this gene and molecular-assisted breeding in maize resistance breeding programs.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (30800688) and PhD Programs Foundation of the Ministry of Education of China (20094105110002).

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Authors and Affiliations

  1. College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China

    Junqiang Ding, Huimin Li, Yongxia Wang, Rongbing Zhao, Xuecai Zhang & Jiafa Chen

  2. College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, China

    Zongliang Xia & Jianyu Wu

Authors
  1. Junqiang Ding
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  2. Huimin Li
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  3. Yongxia Wang
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  4. Rongbing Zhao
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  5. Xuecai Zhang
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  6. Jiafa Chen
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  7. Zongliang Xia
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  8. Jianyu Wu
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Corresponding author

Correspondence to Jianyu Wu.

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Junqiang Ding, Huimin Li authors contributed equally to this paper.

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Ding, J., Li, H., Wang, Y. et al. Fine mapping of Rscmv2, a major gene for resistance to sugarcane mosaic virus in maize. Mol Breeding 30, 1593–1600 (2012). https://doi.org/10.1007/s11032-012-9741-8

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  • DOI: https://doi.org/10.1007/s11032-012-9741-8

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