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Development of SCAR markers linked to a gene controlling absence of tannins in faba bean

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Abstract

Faba beans are inexpensive, nutrient-dense sources of plant protein, but anti-nutritional factors such as condensed tannins reduce the biological value of their protein. Two recessive genes, zt-1 and zt-2, control the absence of tannins in faba bean seeds and also determine a white flower character on the plant. However, crosses between them produce coloured F1 plants with tannins that contaminate the crop. Therefore, it is important to identify the gene present in all tannin-free cultivars and gene bank accessions to enable breeders to choose appropriate genitors for their crosses. The aim of this study was the identification of markers linked to zt-1, one of the genes governing free tannin content in faba bean. A segregating F2 population derived from the cross between the coloured flower and high tannin content genotype Vf6 and a zt-1 line was developed and characterized phenotypically. Bulked Segregant Analysis (BSA) was used to identify Random Amplified Polymorphic DNA (RAPD) markers linked to the zt-1 gene. Four RAPD loci (OPC5551, OPG15600, OPG111171 and OPAF20776) showed polymorphism between the contrasting bulks. The markers were sequenced to develop specific Sequence Characterised Amplified Regions (SCARs). Amplification of SCC5551 produced a single product which was only observed in the white flowered and zero tannin content genotypes, whereas SCAR SCG111171only produced a band in F2 plants with coloured flower and high tannin content. SCARs SCC5551 and SCG111171 were tested for their applicability for routine screening in 37 faba bean genotypes differing in flower colour and tannin content. SCC5551, allowed the prediction of the zt-1 genotypes with a 95% of accuracy, underscoring the potential of this SCAR marker as a cost-effective tool for MAS in large faba bean breeding populations.

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References

  • Avila CM, Sillero JC, Rubiales D, Moreno MT, Torres AM (2003) Identification of RAPD markers linked to the Uvf-1 gene conferring hypersensitive resistance against rust (Uromyces viciae-fabae) in Vicia faba L. Theor Appl Genet 107:353–358

    Article  PubMed  CAS  Google Scholar 

  • Bond DA (1976) In vitro digestibility of the testa in tannin-free field beans (Vicia fabaL). J Agric Sci Camb 86:561–566

    Google Scholar 

  • Bond DA, Poulsen MH (1983) Pollination. In: Hebblethwaite PD (ed) The Faba Bean (Vicia faba L). Butterworths, London, pp 77–101

    Google Scholar 

  • Cabrera A, Martin A (1989) Genetics of tannin content and its relationship with flower and testa colours in Vicia faba L. J Agric Sci Camb 113:93–98

    Article  Google Scholar 

  • Cansfield PE, Marquardt RR, Campbell LD (1980) Condensed proanthocyanidins of faba beans. J Sci Food Agric 31:802–812

    Article  PubMed  CAS  Google Scholar 

  • Cregan PB, Mudge J, Fickus EW, Marek LF, Danesh D, Denny R, Shoemaker RC, Matthews BF, Jarvik T, Young ND (1999) Targeted isolation of simple sequence repeat markers through the use of bacterial artificial chromosomes. Theor Appl Genet 98:918–928

    Article  Google Scholar 

  • Crofton GRA, Bond DA, Duc G (2000) Potential seed multiplication problems arising from the existence of two genes for the absence of tannin in Vicia faba L. Plant Varieties Seeds 13:131–139

    Google Scholar 

  • Chowdhury MA, Andrahennnadi CP, Slinkard AE, Vandenberg A (2001) RAPD and SCAR markers for resistance to ascochyta blight in lentil. Euphytica 118:331–337

    Article  CAS  Google Scholar 

  • Deng Z, Huang S, Xiao S, Gmitter FG (1997) Development and characterization of SCAR markers linked to the citrus tristeza gene from Poncirus trifoliata. Genome 40:697–704

    CAS  PubMed  Google Scholar 

  • Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS (1991) ‘Touch-down’ PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 19:4008

    Article  PubMed  CAS  Google Scholar 

  • Ford R, Pang ECK, Taylor P (1999) Genetics of resistance to Ascochyta blight of lentil and the identification of closely linked RAPD markers. Theor Appl Genet 98:93–98

    Article  CAS  Google Scholar 

  • Garrido A, Gomez-Cabrera A, Guerrero JE, Vandermeer JM (1991) Effects of treatment with polyvinilpirrolidone and polyethylene-glycol on faba bean tannins. Ann Feed Sci Techn 35(3–4):199–203

    Article  CAS  Google Scholar 

  • Gu WK, Weeden NF, Yu J, Wallace DH (1995) Large-scale, costeffective screening of PCR products in marker-assisted selection applications. Theor Appl Genet 91:465–470

    Article  CAS  Google Scholar 

  • Hagerman AE, Butler LG (1978) Protein precipitation method for the quantitative determination of tannins. J Agri Food Chem 26:809–812

    Article  CAS  Google Scholar 

  • Hernández P, Martín A, Dorado G (1999) Development of SCARs by direct sequencing of RAPD products: a practical tool for the introgression and marker-assisted selection of wheat. Mol Breeding 5:245–253

    Article  Google Scholar 

  • Jiménez-Gasco MM, Jiménez-Díaz RM (2003) Development of a specific polymerase chain reaction-based assay for the identification of Fusarium oxysporum f.sp. ciceris and its pathogenic races 0, 1A, 5 and 6. Phytopathology 93:200–209

    Google Scholar 

  • Johnson E, Miklas PN, Stavely JR, Martinez-Cruzado JC (1995) Coupling- and repulsion-phase RAPDs for marker-assisted selection of PI 181996 rust resistance in common bean. Theor Appl Genet 90:659–664

    Article  Google Scholar 

  • Kelly JD (1995) Use of random amplified polymorphic DNA markers in breeding for major gene resistance to plant pathogens. Hort Sci 30:461–465

    Google Scholar 

  • Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer program for constructing genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Marquardt RR (1989) Dietary effects of tannins, vicine and convicine. In: Huisman J, Van der Poel AFB, Liener IE (eds) Recent advances or research in antinutritional factors in legume seeds. Wageningen Academic Publishers, Wageningen, pp 141–155

    Google Scholar 

  • Martin-Tanguy H, Guillaume J, Kossa A (1977) Condensed tannins in horse bean seeds: chemical structure and apparent effects on poultry. J Sci Food Agric 28:757–765

    Article  CAS  Google Scholar 

  • McClendon MT, Inglish DA, McPhee KE, Coyne CJ (2002) DNA markers linked to Fusarium wilt race 1 resistance in pea. J Am Soc Hortic Sci 127(4):602–607

    CAS  Google Scholar 

  • Melotto M, Afanador L, Kelly JD (1996) Development of a SCAR marker linked to the I gene in common bean. Genome 39:1216–1219

    PubMed  CAS  Google Scholar 

  • Michelmore R, Paran I, Keselli V (1991) Identification of markers linked to disease-resistance genes by bulk segregante analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  CAS  Google Scholar 

  • Paran I, Michelmore R (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85:985–993

    Article  CAS  Google Scholar 

  • Picard J (1976) Aperçu sur l’héredité du caractére absence de tannins dans les graines de féverole (Vicia fabaL). Annales de l’Amélioration des Plantes 26:101–106

    Google Scholar 

  • Rafalski JA, Tingey SV, Williams JGK (1991) RAPD markers—a new technology for genetic mapping and plant breeding. AgBiotech News Information 3:645–648

    Google Scholar 

  • Sharp PJ, Johnston S, Brown G, McIntosh RA, Pallotta M, Carter M, Bariana HS, Khartkar S, Lagudah ES, Singh RP, Khairallah M, Potter R, Jones MGK (2001)Validation of molecular markers for wheat breeding. Aust J Agric Res 52:1357–1366

    Article  CAS  Google Scholar 

  • Silva GF, Santos JB, Patto Ramalho MA (2003) Identification of SSR and RAPD markers linked to a resistance allele for angular leaf spot in the common bean (Phaseolus vulgaris) line ESAL 550. Genet Mol Biol 26(4):459–463

    Google Scholar 

  • Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: New tools for an old science. Bio/Technology 7:257–264

    Article  CAS  Google Scholar 

  • Torres AM, Weeden NF, Martín A (1993) Linkage among isozyme, RFLP and RAPD markers in Vicia faba. Theor Appl Genet 85:937–945

    Article  CAS  Google Scholar 

  • Van der Poel AFB, Dellaert LMW, Van Norel A, Helsper JPFG (1992) The digestibility in piglets of faba bean (Vicia faba L) as affected by breeding towards the absence of condensed tannins. Br J Nutr 68:793–800

    Article  PubMed  Google Scholar 

  • Weeden NF (1994) Approaches to mapping in horticultural crops. In: Greshoff PM (ed) Plant genome analysis. CRS Press, Boca Raton FL, pp 57–68

    Google Scholar 

  • Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Article  PubMed  CAS  Google Scholar 

  • Wiseman J, Cole DJA (1988) European legumes in diets for non-ruminants. In: Haresign W, Cole DJA (eds) Recent Advances in Animal Nutrition. Butterworths, London, pp 13–37

    Google Scholar 

  • Yu K, Park S, Poysa V (2000) Marker-assisted selection of common beans for resistance to common bacterial blight: efficacy and economics. Plant Breed 119:411–415

    Article  CAS  Google Scholar 

  • Zhang Y, Stommel JR (2001) Development of SCAR and CAPS markers linked to the beta gene in tomato. Crop Sci 41:1602–1608

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was funded by the grants PETRI 95-0682-OP and INIA RTA04-067 from the Spanish Science and Technology Ministry and by the European Community project EUFABA QLK5-CT2002-02307. N. Gutierrez acknowledges a fellowship from IFAPA-CICE, Junta de Andalucía. CM Avila acknowledges financial support from the Spanish Ministerio de Educación y Ciencia (‘Juan de la Cierva’ program).

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Authors and Affiliations

  1. Area de Mejora y Biotecnología, CIFA “Alameda del Obispo”, IFAPA-CICE, Junta de Andalucía, Apdo. 3092, 14080, Córdoba, Spain

    Natalia Gutierrez, C. Rodriguez-Suarez, M. T. Moreno & A. M. Torres

  2. Departamento de Genética, ETSIAM-UCO, Edificio Mendel (2° Planta), Campus de Rabanales, 14071, Córdoba, Spain

    C. M. Avila

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  1. Natalia Gutierrez
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  2. C. M. Avila
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  3. C. Rodriguez-Suarez
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  4. M. T. Moreno
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  5. A. M. Torres
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Correspondence to Natalia Gutierrez.

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Gutierrez, N., Avila, C.M., Rodriguez-Suarez, C. et al. Development of SCAR markers linked to a gene controlling absence of tannins in faba bean. Mol Breeding 19, 305–314 (2007). https://doi.org/10.1007/s11032-006-9063-9

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  • DOI: https://doi.org/10.1007/s11032-006-9063-9

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