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Extracellular inosine participates in tumor necrosis factor-alpha induced nitric oxide production in cultured Sertoli cells

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Abstract

Recent reports have described purinergic modulation of tumor necrosis factor-alpha (TNF-α) signaling in neutrophils and astrocytes. In Sertoli cells, both TNF-R1 and TNF-R2 TNF-α receptors are present and this cytokine modulates many functions of these cells related to the maintenance of spermatogenesis. Sertoli cells express distinct purinoreceptors and previous work has shown that these cells secrete extracellular nucleotides and their metabolites. In this work, we studied the possible role of extracellular purines in TNF-α signaling in cultured Sertoli cells. This cytokine increased inosine concentration from 30 min to 6 h, with no effect at 24 h. Both TNF-α and inosine increased nitrite accumulation and nitric oxide synthase activity. Erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA), an adenosine deaminase inhibitor, abolished the TNF-α induced inosine increase, nitrite accumulation and nitric oxide synthase activity. These results suggest that extracellular inosine acts as intermediary in TNF-α stimulated nitric oxide production in cultured Sertoli cells.

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Authors and Affiliations

  1. Laboratório de Transdução de Sinais em Células Testiculares, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Luiz Fernando de Souza, Daniel Pens Gelain, Fernanda Rafaela Jardim, Gisele Roncheti Ribeiro, Marcelo Zim & Elena Aida Bernard

  2. Departamento de Bioquímica (ICBS-UFRGS), Rua Ramiro Barcelos, 2600 anexo, CEP 90035-003, Porto Alegre, RS, Brazil

    Elena Aida Bernard

Authors
  1. Luiz Fernando de Souza
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  2. Daniel Pens Gelain
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  3. Fernanda Rafaela Jardim
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  4. Gisele Roncheti Ribeiro
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  5. Marcelo Zim
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  6. Elena Aida Bernard
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Correspondence to Elena Aida Bernard.

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de Souza, L.F., Gelain, D.P., Jardim, F.R. et al. Extracellular inosine participates in tumor necrosis factor-alpha induced nitric oxide production in cultured Sertoli cells. Mol Cell Biochem 281, 123–128 (2006). https://doi.org/10.1007/s11010-006-0639-9

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  • DOI: https://doi.org/10.1007/s11010-006-0639-9

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