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Production of a Recombinant Peptide (Lasioglossin LL ΙΙΙ) and Assessment of Antibacterial and Antioxidant Activity

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Abstract

The use of antimicrobial peptides (AMPs) as biopreservative to replace chemical preservatives has become of interest among consumers. Current approaches of production and purification of AMPs are time-consuming and costly and may kill the producing host cells. In this study a recombinant peptide was patterned from Lasioglossin LL ΙΙΙ which is found naturally in the venom of eusocial bee, this recombinant peptide was produced to inhibit food microbial degradation with the least possible costs. The synthesis of this peptide in the periplasmic space of Escherichia coli, has facilitated the production and purification of this additive. The pET based expression system for production of a recombinant form of Lasioglossin LL ΙΙΙ using E. coli as a host was developed. The antibacterial and antioxidant activities of recombinant peptide were determined. A C-terminal poly histidine tag was used to facilitate purification by standard methods using Ni2+ affinity chromatography. The average yield of the recombinant peptide was 0.7 g/L. The findings showed great antimicrobial function of the peptide. The antioxidant activity of the peptide was lower than Butylated hydroxytoluene (BHT) synthesized antioxidant as a common antioxidant but the antioxidant potential of the peptide along with the antimicrobial activity against foodborne pathogens have shown promising benefits of the peptide. These results demonstrated that pET expression system is appropriate for the rapid and simple isolation of recombinant lasioglossin LL ΙΙΙ from E. coli and the recombinant peptide could be used as a natural preservative source. However, further studies need to be carried out to check the effect of this peptide on more microorganisms.

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Acknowledgements

We hereby acknowledge Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad for providing us with all facilities and financial supports required to perform the practical work.

Funding

This study was funded by the deputy of Research and Technology, Ferdowsi University of Mashhad, Iran (Grant No: 2/45804).

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran

    Abbas Tanhaeian

  2. Department of Food Science & Technology, Ferdowsi University of Mashhad, Mashhad, Iran

    Mohammad B. Habibi Najafi & Paria Rahnama

  3. Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran

    Marjan Azghandi

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  1. Abbas Tanhaeian
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Correspondence to Mohammad B. Habibi Najafi.

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Tanhaeian, A., Habibi Najafi, M.B., Rahnama, P. et al. Production of a Recombinant Peptide (Lasioglossin LL ΙΙΙ) and Assessment of Antibacterial and Antioxidant Activity. Int J Pept Res Ther 26, 1021–1029 (2020). https://doi.org/10.1007/s10989-019-09904-5

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