Abstract
Beneficial effects of MYC such as antioxidant, cytoprotective and antimicrobial activity have been investigated in various studies. The aim of the present research was to evaluate the poisonous impact of myricetin (MYC) that have been encapsulated in solid lipid nanoparticles (MYC-SLNs) on the growth of A549 human lungs adenocarcinoma cancer cells. A549 and MRC5 (healthy cells) cell lines were given with 25 μM of MYC or MYC-SLNs for 24 h and cell viability, colony formation, and gene expression were evaluated. The MYC-SLNs with appropriate characteristics (particle size of 89.23 ± 6.9 nm, Zeta potential of − 27.9 and entrapment efficiency of 69.2%) were prepared. Cytotoxicity evaluations demonstrated inhibition of the growth of A549 cells of a little IC50 (50% suppressive concentration) value via MYC-SLNs. Induction of a considerable decline of rapid growth and viability of A549 cells has been performed by MYC-SLNs. While a slight increase in the percentage of apoptosis was observed in the A549 cells, necrosis percentage was significantly increased by MYC-SLNs in comparison with the free MYC. Results showed that MYC-SLNs could upregulate the expression of necroptotic related genes such as RIPK3 and MLKL in the A549. MYC-SLNs or MYC did not influence proliferation, apoptosis, and viability of MRC5 cells. With regard to the findings, SLNs considerably augmented the poisonous impact of MYC versus cancer cells of human lungs.
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The work described in this paper is a part of Ph.D. thesis of Atefeh Ashtari and was substantially supported by the Research Council of Ahvaz Jundishapur University of Medical Sciences, which are gratefully acknowledged.
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The study was supported by Grant CMRC-9623 from the Research Council of Ahvaz Jundishapur University of Medical Sciences.
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Layasadat Khorsandi, Esrafil Mansouri, Mohammad Rashno, Masoud Ali Karami and Atefeh Ashtari declare that they have no conflict of interest.
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Khorsandi, L., Mansouri, E., Rashno, M. et al. Myricetin Loaded Solid Lipid Nanoparticles Upregulate MLKL and RIPK3 in Human Lung Adenocarcinoma. Int J Pept Res Ther 26, 899–910 (2020). https://doi.org/10.1007/s10989-019-09895-3
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DOI: https://doi.org/10.1007/s10989-019-09895-3