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Anticancer Activities of Antimicrobial BmKn2 Peptides Against Oral and Colon Cancer Cells

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International Journal of Peptide Research and Therapeutics Aims and scope Submit manuscript

Abstract

There is considerable current interest in developing antimicrobial and anticancer agents with a new mode of action. The antimicrobial peptides are regarded as a potential solution for treating cancer cells. The antimicrobial effect of 6 synthetic peptides against 7 bacterial species was evaluated. The result showed that IsCT, BmKn2 and BMAP-28 exhibited broad range of action against Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538, methicillin resistant S. aureus DMST 20651, Staphylococcus epidermidis ATCC 12228, Acinetobacter baumanii ATCC 19066, Escherichia coli ATCC 25922 and Salmonella typhi DMST 562 at minimal inhibitory concentrations (MIC) of 2.97–24.28 μM. Neither AMP induced significant hemolysis, or showed cytotoxic on dental pulp stem cells and smooth muscle cells at their MICs. In addition, BmKn2 inhibited growth of human oral squamous carcinoma HSC4 cells and human colon cancer SW620 cells with IC50 of 17.26 and 40 µM, respectively. Taken together, BmKn2 peptide from scorpion venom may offer a novel therapeutic strategy for development of cationic antimicrobial and anticancer peptides as potential new therapeutic agents.

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Acknowledgments

We would like to thank TU Research grant supported by Thammasat University for the financial support to this study. Thanks are also extended to Faculty of Science, Ramkhamhaeng University and Faculty of Dentist, Mahidol University for kind supports in providing SW620 cell line, oral cancer cell line and dental pulp stem cells.

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Authors and Affiliations

  1. Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, Thailand

    Teerakul Arpornsuwan & Wimolpak Sriwai

  2. Genome Institute, National Center for Genetic Engineering and Biotechnology, Thailand Science Park, Pathumthani, Thailand

    Janthima Jaresitthikunchai, Narumon Phaonakrop & Sittiruk Roytrakul

  3. Faculty of Dentistry, Mahidol University, Bangkok, Thailand

    Hathaitip Sritanaudomchai

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  1. Teerakul Arpornsuwan
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  2. Wimolpak Sriwai
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Correspondence to Teerakul Arpornsuwan.

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Arpornsuwan, T., Sriwai, W., Jaresitthikunchai, J. et al. Anticancer Activities of Antimicrobial BmKn2 Peptides Against Oral and Colon Cancer Cells. Int J Pept Res Ther 20, 501–509 (2014). https://doi.org/10.1007/s10989-014-9417-9

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  • DOI: https://doi.org/10.1007/s10989-014-9417-9

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