The overall objective of our research is to produce polyanion/chitosan nanoparticulate oral delivery systems for insulin. Specific objectives of the present study were to study dextran sulfate or alginate complexation with chitosan on mean particle size, insulin association efficiency, loading capacity and release profile. Nanoparticles were formed by ionotropic complexation and coacervation between polyanions (dextran sulfate and alginate) and chitosan. Diameter was evaluated with photon correlation spectroscopy, polymer interaction was confirmed by DSC and FTIR and particle morphology was assessed by SEM and TEM. Mean nanoparticle diameter ranged from 423 to 850 nm, insulin association efficiency from 63 to 94% and loading capacity from 5 to 13%. Dextran sulfate provided highest insulin association efficiency and retention of insulin in gastric simulated conditions. These nanoparticle systems show promise as insulin and potentially other therapeutic polypeptides carriers.
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Acknowledgements
This work was supported by Fundação para a Ciência e Tecnologia, Portugal and the Natural Sciences and Engineering Research Council of Canada. The authors wish to thank Lilly Farma for insulin supply.
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Sarmento, B., Martins, S., Ribeiro, A. et al. Development and Comparison of Different Nanoparticulate Polyelectrolyte Complexes as Insulin Carriers. Int J Pept Res Ther 12, 131–138 (2006). https://doi.org/10.1007/s10989-005-9010-3
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DOI: https://doi.org/10.1007/s10989-005-9010-3