Skip to main content
SpringerLink
Log in

Thermochemical, volumetric and spectroscopic properties of lysozyme–poly(ethylene) glycol system

  • Published:
Journal of Thermal Analysis and Calorimetry Aims and scope Submit manuscript

Abstract

Investigations of lysozyme–polyethylene glycol system were made by differential scanning calorimetry, fluorescence and density techniques. The values of unfolding enthalpies, ΔH N U, unfolding temperatures, T m, excess molar heat capacities, ΔC p, and apparent molar volumes, V Φ , were determined as functions of PEG concentration. The three PEGs of average molecular mass (MW) 6000, 10000, 20000 were used as macromolecular crowding agents. The concentration of polymers was changed in the range 0–30% mass per volume (w/v). The values of ΔH N U remained constant with no dependence on PEG concentration, while PEG addition to buffered lysozyme solutions caused linear decrease of T m. The values of ΔC p and V Φ of lysozyme dramatically changed in the range of 8–10% of PEG concentration. The fluorescence spectroscopy was used in order to investigate the polymer influence on possible solvent–lysozyme interactions. The electrical properties of polymer–water and polymer–buffer systems, the dielectric constants of solutions were determined with use of impedance spectroscopy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. CR Middaugh WA Tisel RN Haire A Rosenberg (1978) J. Biol. Chem. 254 367

    Google Scholar 

  2. TC Laurent (1971) Eur. J. Biochem. 21 498 Occurrence Handle1:CAS:528:DyaE3MXltVGnurg%3D Occurrence Handle10.1111/j.1432-1033.1971.tb01495.x

    Article  CAS  Google Scholar 

  3. JM Harris (1992) Poly(Ethylene Glycol) Chemistry: Biotechnical and Biomedical Applications Plenum Press New York Occurrence Handle10.1007/978-1-4899-0703-5

    Book  Google Scholar 

  4. S Verheyen N Blaton R Kinget G van den Mooter (2004) J. Therm. Anal. Cal. 76 405 Occurrence Handle1:CAS:528:DC%2BD2cXktVWhurg%3D Occurrence Handle10.1023/B:JTAN.0000028020.02657.9b

    Article  CAS  Google Scholar 

  5. SB Zimmerman AP Minton (1993) Annu. Rev. Biomol. Struct 22 27 Occurrence Handle1:CAS:528:DyaK3sXlsFCjurg%3D Occurrence Handle10.1146/annurev.bb.22.060193.000331

    Article  CAS  Google Scholar 

  6. RJ Ellis AP Minton (2003) Nature 425 27 Occurrence Handle1:CAS:528:DC%2BD3sXmvFaguro%3D Occurrence Handle10.1038/425027a

    Article  CAS  Google Scholar 

  7. RJ Ellis (2001) Trends in Biochem. Sci. 26 597 Occurrence Handle1:CAS:528:DC%2BD3MXnt1Wjsbc%3D Occurrence Handle10.1016/S0968-0004(01)01938-7

    Article  CAS  Google Scholar 

  8. RJ Ellis (2001) Current Op. Struct. Biol. 11 114 Occurrence Handle1:CAS:528:DC%2BD3MXhs1Kis7o%3D Occurrence Handle10.1016/S0959-440X(00)00172-X

    Article  CAS  Google Scholar 

  9. LL-Y Lee JC Lee (1987) Biochemistry 26 7831

    Google Scholar 

  10. A Malzert F Boury D Renard P Robert L Lavenant JP Benoit JE Proust (2003) Int. J. Pharm. 260 175 Occurrence Handle1:CAS:528:DC%2BD3sXkvFekt7g%3D Occurrence Handle10.1016/S0378-5173(03)00258-8

    Article  CAS  Google Scholar 

  11. DA Knoll J Hermans (1981) Biopolymers 20 1747 Occurrence Handle1:CAS:528:DyaL3MXlt1Oksbg%3D Occurrence Handle10.1002/bip.1981.360200814

    Article  CAS  Google Scholar 

  12. DH Atha KC Ingham (1981) J. Biol. Chem. 256 12108 Occurrence Handle1:CAS:528:DyaL3MXmtFOhu7o%3D

    CAS  Google Scholar 

  13. K Sasahara P McPhe AP Minton (2003) J. Mol. Biol. 326 1227 Occurrence Handle1:CAS:528:DC%2BD3sXhtVCiurg%3D Occurrence Handle10.1016/S0022-2836(02)01443-2

    Article  CAS  Google Scholar 

  14. B Ferruggia B Nerli H Di Nuci R Rigatusso G Pico (1999) Int. J. Biol.Macromol. 26 23 Occurrence Handle10.1016/S0141-8130(99)00061-6

    Article  Google Scholar 

  15. PL Privalov SA Potekhin (1986) Methods Enzymol. 131 4 Occurrence Handle1:CAS:528:DyaL2sXmtlOisQ%3D%3D Occurrence Handle10.1016/0076-6879(86)31033-4

    Article  CAS  Google Scholar 

  16. VL Shnyrov JM Sanchez-Ruiz BN Boiko GG Zhadan EA Permyakov (1997) Thermochim. Acta 302 165 Occurrence Handle10.1016/S0040-6031(97)00238-4

    Article  Google Scholar 

  17. PL Privalov NN Khechinashvili (1974) J. Mol. Biol. 86 665 Occurrence Handle1:CAS:528:DyaE2MXlt12gsA%3D%3D Occurrence Handle10.1016/0022-2836(74)90188-0

    Article  CAS  Google Scholar 

  18. W Pfeil PL Privalov (1976) Biophys. Chem. 4 23 Occurrence Handle1:CAS:528:DyaE28XhtFOktrw%3D Occurrence Handle10.1016/0301-4622(76)80003-8

    Article  CAS  Google Scholar 

  19. PL Privalov GI Makhatadze (1990) J. Mol. Biol. 213 385 Occurrence Handle1:CAS:528:DyaK3cXksFClsb4%3D Occurrence Handle10.1016/S0022-2836(05)80198-6

    Article  CAS  Google Scholar 

  20. VV Plotnikov JM Brandts LN Lin JF Brandts (1997) Anal. Biochem. 250 237 Occurrence Handle1:CAS:528:DyaK2sXltlWgsr0%3D Occurrence Handle10.1006/abio.1997.2236

    Article  CAS  Google Scholar 

  21. A Michnik A Kołos Z Drzazga (2004) J. Therm. Anal. Cal. 77 269 Occurrence Handle1:CAS:528:DC%2BD2cXlt1ynsLo%3D Occurrence Handle10.1023/B:JTAN.0000033212.93809.e1

    Article  CAS  Google Scholar 

  22. HJ Hinz FP Schwarz (2001) Pure Appl. Chem. 73 745 Occurrence Handle1:CAS:528:DC%2BD3MXltVyks7w%3D Occurrence Handle10.1351/pac200173040745

    Article  CAS  Google Scholar 

  23. K Arnold A Hermann L Pratsch K Gawrisch (1985) BBA 815 515 Occurrence Handle1:CAS:528:DyaL2MXkvVekurs%3D Occurrence Handle10.1016/0005-2736(85)90381-5

    Article  CAS  Google Scholar 

  24. P. L. Mateo, Differental scanning calorimetry applications to protein solutions. Thermochemistry and its Applications to Chemical and Biochemical Systems, (1984), pp. 341–368.

  25. AD Robertson KP Murphy (1997) Chem. Rev. 97 1251 Occurrence Handle1:CAS:528:DyaK2sXksFSisLY%3D Occurrence Handle10.1021/cr960383c

    Article  CAS  Google Scholar 

  26. SN Timasheff GD Fasman (1969) Structure and Stability of Biological Macromolecules Marcel Dekker Inc. New York

    Google Scholar 

  27. SN Timasheff (1993) Annu. Rev. Biophys. Biomol. Struct. 22 67 Occurrence Handle1:CAS:528:DyaK3sXlsFCjurk%3D Occurrence Handle10.1146/annurev.bb.22.060193.000435

    Article  CAS  Google Scholar 

  28. TV Chalikian (2003) Annu. Rev. Biophys. Biomol. Struct. 32 207 Occurrence Handle1:CAS:528:DC%2BD3sXntFSgtr8%3D Occurrence Handle10.1146/annurev.biophys.32.110601.141709

    Article  CAS  Google Scholar 

  29. AJ Sophianopoulos CKJ Rhode DN Holcomb KE Van Holde (1962) J. Biol. Chem. 237 1107 Occurrence Handle1:CAS:528:DyaF3sXpvVOq

    CAS  Google Scholar 

  30. C Tanford (1961) Physical Chemistry of Macromolecules J. Wiley New York

    Google Scholar 

  31. TE Creighton (1992) Proteins: structure and molecular properties W. H. Freeman and company New York

    Google Scholar 

  32. A Cao G Wang Y Tang L Lai (2002) Biochem. Biophys. Res. Com. 291 795 Occurrence Handle1:CAS:528:DC%2BD38XhsFWjs7c%3D Occurrence Handle10.1006/bbrc.2002.6526

    Article  CAS  Google Scholar 

  33. M Hirai S Arai H Iwase T Takizawa (1998) J. Phys. Chem. B 102 1308 Occurrence Handle1:CAS:528:DyaK1cXls1arug%3D%3D Occurrence Handle10.1021/jp9713367

    Article  CAS  Google Scholar 

  34. DH Atha KC Ingham (1981) J. Biol. Chem. 256 12108 Occurrence Handle1:CAS:528:DyaL3MXmtFOhu7o%3D

    CAS  Google Scholar 

  35. J Hermans (1982) J. Chem. Phys. 77 2193 Occurrence Handle1:CAS:528:DyaL38Xlt1agurs%3D Occurrence Handle10.1063/1.444026

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Physical Chemistry of the Polish Academy of Sciences, Warsaw, Poland, 01-224, Kasprzaka 44/52

    Zielenkiewicz W & Swierzewski R

  2. Institute for Macromolecular Studies – Division of Genova, CNR, Genova, Italy, 16-149, Via De Marini 6

    Attanasio F & Rialdi G

Authors
  1. Zielenkiewicz W
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Swierzewski R
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Attanasio F
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rialdi G
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zielenkiewicz W.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zielenkiewicz, W., Swierzewski, R., Attanasio, F. et al. Thermochemical, volumetric and spectroscopic properties of lysozyme–poly(ethylene) glycol system. J Therm Anal Calorim 83, 587–595 (2006). https://doi.org/10.1007/s10973-005-7417-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10973-005-7417-x

Keywords

Search

Navigation