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Urease adsorption and activity on magnetite nanoparticles functionalized with monofunctional and bifunctional surface layers

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Abstract

The surface of magnetite nanoparticles was coated with functional polysiloxane layers using reaction of hydrolytic copolycondensation of tetraethoxysilane and 3-aminopropyltriethoxysilane (or N-[3-trimethoxysilylpropyl]ethylendiamine), and also that of tetraethoxysilane, 3-aminopropyltriethoxysilane and methyltriethoxysilane (or n-propyltriethoxysilane). It was shown that these functionalized magnetically controllable particles (about 60–150 nm in size as aggregates), as opposed to magnetite, adsorb urease well from aqueous solutions (up to 1 g/g), and that the level of residual activity of adsorbed layers is up to 84 % in the case of a bifunctional sample. It was established that the activity of immobilized urease is normally gradually reduced during storage of the samples, but in the case of ethylenediamine functional group is not decreased for 45 days. The synthesized samples are promising for use as magnetically directed biocatalysts.

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Acknowledgments

R. P. P., I. V. M. and Yu. L. Z. would like to thank the State Target Scientific and Technical Program “Nanotechnologies and Nanomaterials” (project 6.22.5.42) and TCPFR “Fundamental Problems of Nanostructural Systems, Nanomaterials, and Nanotechnologies” (project no. 57/12-H) of NAS of Ukraine. V. G. K. and G. A. S. express their gratitude to the Swedish Research Council for support of the project “Molecular Precursors and Molecular Models of Nanoporous Materials” and to the EU FP7 program for support of the EuRARE project.

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Authors and Affiliations

  1. Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, 17, General Naumov Street, Kyiv, 03164, Ukraine

    Roman P. Pogorilyi, Inna V. Melnyk & Yuriy L. Zub

  2. Deparment of Chemistry, Swedish University of Agricultural Sciences, 750 07, Uppsala, Sweden

    Guliam A. Seisenbaeva & Vadim G. Kessler

  3. Kholodnyi Institute of Botany, National Academy of Sciences of Ukraine, 2, Tereshchenkivska Street, Kyiv, 01601, Ukraine

    Mykola M. Shcherbatyik

  4. Faculty of Mechanical Engineering, Centre for Sensor Technology, University of Maribor, Smetanova 17, 2000, Maribor, Slovenia

    Aljoša Košak & Aleksandra Lobnik

Authors
  1. Roman P. Pogorilyi
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  2. Inna V. Melnyk
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  3. Yuriy L. Zub
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  4. Guliam A. Seisenbaeva
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  5. Vadim G. Kessler
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  6. Mykola M. Shcherbatyik
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  8. Aleksandra Lobnik
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Correspondence to Inna V. Melnyk or Yuriy L. Zub.

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Pogorilyi, R.P., Melnyk, I.V., Zub, Y.L. et al. Urease adsorption and activity on magnetite nanoparticles functionalized with monofunctional and bifunctional surface layers. J Sol-Gel Sci Technol 68, 447–454 (2013). https://doi.org/10.1007/s10971-013-2991-z

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  • DOI: https://doi.org/10.1007/s10971-013-2991-z

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