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Synthesis, characterization, toxicity and in vivo imaging of lysine graft polymeric nanoparticles

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Abstract

Polymeric nanoparticles are commonly used in several biological applications. There are limited number of studies on the toxicity and potential effectiveness of polymeric nanoparticles to need for further study on polymer science. The aim of the study was to investigate characterization, in vitro, in vivo toxicity and biological distribution of Lys-graft-p(HEMA) polymeric nanoparticle. The characterization analyses showed that Lys-graft-p(HEMA) had an average size of around 171 nm and zeta potential was −22.6 mV. The sample was recorded from 750 to 4000 cm−1 in FTIR spectroscopy and the characteristic peaks of stretching band were observed in the spectrum. The polymeric nanoparticle did not show any cytotoxic effect on human embryonic kidney 293 healthy cell line (HEK 293, ATCC-CRL-1573) after 24 and 48 h of exposure. The nanoparticle did not cause mutation effects on Salmonella typhimurium TA 98, TA 100, TA 1535 and TA 1537 strains. In addition, it has not hemolitic activity on rabbit erythrocytes at applied concentration and no lethality was observed with single oral dose toxicity test. FITC-Lys-graft-p(HEMA) were observed on different organs such as liver, kidney, heart, lung and large intestine at 6. hours by in vivo imaging system. These results reveal that Lys-graft-p(HEMA) polymeric nanoparticles can be used as a potential drug carrier system because of its biocompatibility.

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Acknowledgements

Characterization analyses and experimental studies on animals were performed in Ege University, Drug Research and Development and Pharmacokinetic Applications (ARGEFAR).

Funding

The study is supported by Ege University BAP project (2015/ FEN/010).

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Authors and Affiliations

  1. Faculty of Science, Department of Biology, Ege University, 35100, Bornova, Izmir, Turkey

    Buket Bakan, Cinel Koksal Karayildirim, Melih Dagdeviren & N. Ulku Karabay Yavasoglu

  2. Vocational School, Medical Services and Techniques, Beykent University, 34398, Sarıyer Istanbul, Turkey

    Ceren Turkcan Kayhan

  3. Faculty of Science, Department of Chemistry, Ege University, 35100, Bornova, Izmir, Turkey

    Suleyman Gulcemal & Yeliz Yildirim

  4. Faculty of Science, Department of Biochemistry, Ege University, 35100, Bornova, Izmir, Turkey

    Sinan Akgol

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  1. Buket Bakan
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Bakan, B., Kayhan, C.T., Koksal Karayildirim, C. et al. Synthesis, characterization, toxicity and in vivo imaging of lysine graft polymeric nanoparticles. J Polym Res 26, 239 (2019). https://doi.org/10.1007/s10965-019-1901-7

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