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The Histone H1 Variant Accumulates in Response to Water Stress in the Drought Tolerant Genotype of Gossypium herbaceum L.

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Abstract

We have optimized and improved the protocol for extraction of histone proteins from Gossypium herbaceum. Histone proteins were isolated by acid extraction method and fractionation of histone proteins were performed using RP-HPLC (reverse-phase high performance liquid chromatography). Analysis of histones from drought tolerant (Vagad) and drought sensitive genotype (RAHS-14) indicated that the tolerant genotype Vagad encodes a 29 kDa protein. Protein sequencing on MALDI TOF/TOF revealed that the 29 kDA protein shared sequence similarity with another drought-inducible linker histone-H1.S reported in tomato. This H1.S like linker histone was not found in RAHS-14 in our study. We further examined the expression of H1 variant at the transcript and protein levels and found that it was induced specifically in the tolerant genotype Vagad.

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Abbreviations

RP-HPLC:

Reverse-phase high performance liquid chromatography

w/v:

Weight/volume

Tris:

Tris (hydroxymethyl) aminomethane

EDTA:

Ethylenediaminetetraacetic acid

SDS-PAGE:

Sodium dodecyl sulphate–polyacrylamide gel electrophoresis

PVDF:

Polyvinylidene difluoride

MALDI-TOF:

Matrix-assisted laser desorption/ionization- time-of-flight

RT-PCR:

Real-time PCR

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Acknowledgments

This work was supported by a research grant received from the Council of Scientific and Industrial Research (CSIR), Government of India. IT and AR acknowledge CSIR for research fellowship.

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Authors and Affiliations

  1. Plant Molecular Biology and Genetic Engineering Division, CSIR, National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226001, UP, India

    Ila Trivedi, Alok Ranjan & Samir Sawant

  2. Department of Botany, Faculty of Science, University of Lucknow, Lucknow, 226007, India

    Y. K. Sharma

Authors
  1. Ila Trivedi
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  2. Alok Ranjan
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  3. Y. K. Sharma
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  4. Samir Sawant
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Correspondence to Samir Sawant.

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Trivedi, I., Ranjan, A., Sharma, Y.K. et al. The Histone H1 Variant Accumulates in Response to Water Stress in the Drought Tolerant Genotype of Gossypium herbaceum L.. Protein J 31, 477–486 (2012). https://doi.org/10.1007/s10930-012-9425-6

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