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Multi-Functional Carbon Dots from an Ayurvedic Medicinal Plant for Cancer Cell Bioimaging Applications

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Abstract

The combination of an Ayurvedic wisdom and nanotechnology may help us to resolve the complex healthcare challenges. A facile and economical one-pot hydrothermal synthesis method has been adopted for preparing a blue fluorescent carbon dots (CDs) with a quantum yield of 15.10% from an Ayurvedic medicinal plant Andrographis paniculata (AP). The Andrographis paniculata derived CDs (AAPCDs) were then characterized using different techniques. Through High Performance Thin Layer Chromatography (HPTLC) profiling of the AP extract and the CDs, it was found that some of the phytoconstituents are retained as such while others may have been converted into their derivatives during the process of formation of CDs. The CDs are designed to possess cellular imaging of human breast carcinoma cells (MCF-7), apart from free radicals sensing and scavenging capabilities. AAPCDs showed minimal cytotoxicity in Multi Drug Resistant clinically isolated strains of gram positive and gram negative bacteria which may be employed for microbiology oriented experiments. These results suggest potential of multi-functional AAPCDs as nano-probes for various pharmaceutical, biomedical and bioengineering applications.

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Acknowledgments

The authors would like to thank Ministry of Human Resource Development (MHRD), New Delhi, Central Instrumentation Facility, IIT (BHU) and the Indian Institute of Technology (BHU) for their support. The authors would also like to thank the Department of Science and Technology (DST-SERB), India-SB/S2/RJN-140/2014 (Ramanujan Fellowship Award) for the financial support of this study.

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Authors and Affiliations

  1. Department of Pharmaceutical Engineering & Technology, IIT (BHU), Varanasi, 221005, India

    Gaurav Gopal Naik, Debadatta Mohapatra & Alakh N. Sahu

  2. Department of Physics, IIT (BHU), Varanasi, 221005, India

    Md. Bayazeed Alam & Avanish S. Parmar

  3. Centre for Genetics Disorders, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India

    Vivek Pandey & Pawan K. Dubey

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  1. Gaurav Gopal Naik
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  2. Md. Bayazeed Alam
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Correspondence to Alakh N. Sahu.

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Naik, G.G., Alam, M.B., Pandey, V. et al. Multi-Functional Carbon Dots from an Ayurvedic Medicinal Plant for Cancer Cell Bioimaging Applications. J Fluoresc 30, 407–418 (2020). https://doi.org/10.1007/s10895-020-02515-0

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  • DOI: https://doi.org/10.1007/s10895-020-02515-0

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