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Fluorescence Modulation of Acridine and Coumarin Dyes by Silver Nanoparticles

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Abstract

Silver nanoparticles were synthesized by chemical reduction of silver ions by sodium borohydride in the presence of poly-(N)-vinyl-2-pyrrolidone in solution of short chain alcohols. The nanoparticles are stable in 2-propanol, and the average diameter of the Ag colloid obtained in this solvent is about 6 nm. The photophysical properties of acridinium and coumarin dyes in 2-propanol are affected by the presence of silver nanoparticles. The interaction of silver nanoparticles with acridinium derivative leads to a spectral change of its intramolecular charge transfer (ICT) absorption band. The dye emission increases suddenly with the initial addition of the Ag metal nanoparticles, but at a high concentration of the colloid, static fluorescence quenching occurs with a progressive decrease of the fluorescence efficiency. Amino coumarin fluorescence is only quenched by the silver nanoparticles in solution.

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References

  1. Kamat PV (2002) Photophysical, photochemical and photocatalytic aspects of metal nanoparticles. J Phys Chem B 106:7729–7744

    Article  CAS  Google Scholar 

  2. Lakowicz JR (2001) Radiative decay engineering. Anal Biochem 298:1–24

    Article  PubMed  CAS  Google Scholar 

  3. Lakowicz JR, Shen Y, D’Auria S, Malicka J, Fang J, Gryczynski Z, Gryczynski I (2002) Radiative decay engineering: 2. Effects of silver islands films on fluorescence intensity, lifetimes, and resonance energy transfer. Anal Biochem 301:261–277

    Article  PubMed  CAS  Google Scholar 

  4. Lakowicz JR (2004) Radiative decay engineering 3. Surface plasmon-coupled directional emission. Anal Biochem 324:153–169

    Article  PubMed  CAS  Google Scholar 

  5. Aslan K, Cryczynski I, Malicka J, Lakowicz JR, Geddes CD (2005) Metal-enhanced fluorescence: an emerging tool in biotechnology. Curr Opin Biotechnol 16(1):55–62

    Article  PubMed  CAS  Google Scholar 

  6. Aslan K, Holley P, Davies L, Lakowicz JR, Geddes CD (2005) Angular-ratiometric plasmon-resonance based light scattering for bioaffinity. J Am Chem Soc 127:12115–12121

    Article  PubMed  CAS  Google Scholar 

  7. Aslan K, Malyn SN, Geddes CD (2007) Metal-enhanced fluorescence from gold surfaces: angular dependent emission. J Fluoresc 17:7–13

    Article  PubMed  CAS  Google Scholar 

  8. Zhang J, Matveeva E, Gryczynski I, Leonenko Z, Lakowicz JR (2005) Metal-enhanced fluoroimmunoassay on a silver film by vapor deposition. J Phys Chem B 109:7969–7975

    Article  PubMed  CAS  Google Scholar 

  9. Zhang J, Malicka J, Gryczynski I, Lakowicz JR (2005) Surface-enhanced fluorescence of fluorescein-labeled oligonucleotides capped on silver nanoparticles. J Phys Chem B 109:7643–7648

    Article  PubMed  CAS  Google Scholar 

  10. Corrigan TD, Guo S, Phaneuf RJ, Szmacinski H (2005) Enhanced fluorescence from periodic arrays of silver nanoparticles. J Fluoresc 15:777–784

    Article  PubMed  CAS  Google Scholar 

  11. Haes AJ, Stuart DA, Nie S, Van Duyne RP (2004) Using solution-phase nanoparticles, surface-confined nanoparticle arrays and single nanoparticles as biological sensing platforms. J Fluoresc 14:355–367

    Article  PubMed  CAS  Google Scholar 

  12. Machulek Junior A, De Oliveira HPM, Gehlen MH (2003) Preparation of silver nanoprisms using poly(N-vinyl-2-pyrrolidone) as a colloid stabilizing agent and the effect of silver nanoparticles on the photophysical properties of cationic dyes. Photochem Photobiol Sci 2:921–925

    Article  PubMed  CAS  Google Scholar 

  13. Zhao SY, Chen SH, Li DG, Yang XG, Ma HY (2004) A convenient phase transfer route for Ag nanoparticles. Physica E 23:92–96

    Article  CAS  Google Scholar 

  14. Kumar A, Joshi H, Pasricha R, Mandale AB, Sastry M (2003) Phase transfer of silver nanoparticles from aqueous to organic solutions using fatty amine molecules. J Colloid Interface Sci 264:396–401

    Article  PubMed  CAS  Google Scholar 

  15. Pereira RV, Ferreira APG, Gehlen MH (2005) Excited-state intramolecular charge transfer in 9-aminoacridine derivative. J Phys Chem A 109:5978–5983

    Article  PubMed  CAS  Google Scholar 

  16. Pereira RV, Gehlen MH (2006) Photoinduced intramolecular charge transfer in 9-aminoacridinium derivatives assisted by intramolecular H-bond. J Phys Chem A 110:7539–7546

    Article  PubMed  CAS  Google Scholar 

  17. Hao E, Schatz GC, Hupp JT (2004) Synthesis and optical properties of anisotropic metal nanoparticles. J Fluoresc 14:331–341

    Article  PubMed  CAS  Google Scholar 

  18. Haykawa T, Selvan ST, Nogami M (1999) Enhanced fluorescence from Eu+3 owing to surface plasma oscillation of silver particles in glass. J Non-Cryst Solids 259:16–22

    Article  Google Scholar 

  19. Lakowicz JR (1999) Principles of fluorescence spectroscopy, 2nd edn. Kluwer, New York, chapter 8

    Google Scholar 

  20. Gehlen MH, De Schryver FC (1993) Time resolved fluorescence quenching in micellar assemblies. Chem Rev 93:199–221

    Article  CAS  Google Scholar 

  21. Barik A, Kumbhakar M, Nath S, Pal H (2005) Evidence for the TICT mediated nonradiative deexcitation process for the excited coumarin-1 dye in high polarity protic solvents. Chem Phys 315:277–285

    Article  CAS  Google Scholar 

  22. Das H, Jain B, Patel HS (2006) Hydrogen bonding properties of coumarin 151, 500 and 35: the effect of substitution at 7-amino position. J Phys Chem A 110:1698–1704

    Article  PubMed  CAS  Google Scholar 

  23. Rivas L, Murza A, Sánchez-Cortés S, García-Ramos JV (2001) Adsorption of acridine drugs on silver surface-enhanced resonance Raman evidence of the existence of different adsorption sites. Vibr Spectrosc 25:19–28

    Article  CAS  Google Scholar 

  24. Aslan K, Leonenko Z, Lakowicz JR, Geddes CD (2005) Annealed silver-island films for applications in metal-enhanced fluorescence: interpretation in terms of radiating plasmons. J Fluoresc 15:643–654

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Instituto de Química de São Carlos, Universidade de São Paulo, 13566-590, São Carlos, SP, Brazil

    Carolina A. Sabatini, Robson V. Pereira & Marcelo H. Gehlen

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  1. Carolina A. Sabatini
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  2. Robson V. Pereira
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  3. Marcelo H. Gehlen
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Correspondence to Marcelo H. Gehlen.

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Sabatini, C.A., Pereira, R.V. & Gehlen, M.H. Fluorescence Modulation of Acridine and Coumarin Dyes by Silver Nanoparticles. J Fluoresc 17, 377–382 (2007). https://doi.org/10.1007/s10895-007-0204-2

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  • DOI: https://doi.org/10.1007/s10895-007-0204-2

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