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Microwave-Accelerated Metal-Enhanced Fluorescence (MAMEF): Application to Ultra Fast and Sensitive Clinical Assays

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In this rapid communication we describe an exciting platform technology that promises to fundamentally address two underlying constraints of modern assays and immunoassays, namely sensitivity and rapidity. By combining the use of Metal-enhanced Fluorescence (MEF) with low power microwave heating (Mw), we can significantly increase the sensitivity of surface assays as well as >95% kinetically complete the assay within a few seconds. This technology is subsequently likely to find significant importance in certain clinical assays, such as in the clinical assessment of myoglobin, where both the assay rapidity and sensitivity are paramount for the assessment and treatment of acute myocardial infarction.

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Abbreviations

AFM, Atomic Force Microscopy; BSA, Bovine Serum Albumin; MAMEF, Microwave-Accelerated Metal-Enhanced Fluorescence; MEF, Metal-Enhanced Fluorescence; Mw:

Low-Power Microwave exposure; RDE, Radiative Decay Engineering; SiFs: Silver Island Films.

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ACKNOWLEDGMENTS

This work was supported by the NIH GM070929 and the National Center for Research Resources, RR008119. Partial salary support to CDG from UMBI/MBC is also acknowledged.

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Authors and Affiliations

  1. Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard St., Baltimore, Maryland, U.S.

    Kadir Aslan & Chris D. Geddes

  2. Center for Fluorescence Spectroscopy, Medical Biotechnology Center, University of Maryland School of Medicine, 725 West Lombard St., Baltimore, Maryland., U.S.

    Chris D. Geddes

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  1. Kadir Aslan
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  2. Chris D. Geddes
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Correspondence to Chris D. Geddes.

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Aslan, K., Geddes, C.D. Microwave-Accelerated Metal-Enhanced Fluorescence (MAMEF): Application to Ultra Fast and Sensitive Clinical Assays. J Fluoresc 16, 3–8 (2006). https://doi.org/10.1007/s10895-005-0026-z

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  • DOI: https://doi.org/10.1007/s10895-005-0026-z

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