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Production, Optimization and Characterisation of Chitosanase of Bacillus sp. and its Applications in Nanotechnology

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Abstract

Chitosanases is a class of enzymes which hydrolyse chitosan, a natural biopolymer consisting of d-glucosamine in various degrees. In this study, chitosanase producing Bacillus sp. was isolated from soil sample. Chitosanase production was optimized using response surface methodology and the produced chitosanase was characterized. The crude enzyme was found to possess antibacterial and antifungal activity. Chitosanase enzyme was used for trimming chitosan based polymeric nanoparticles produced using sodium trimetaphosphate chelator. Chitosanase enzyme was also utilized for synthesis of silver nanoparticles which were then characterized by UV–Vis, FTIR, SEM, TEM and AFM. The produced nanoparticles were checked for antibacterial and antifungal activity.

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Authors and Affiliations

  1. Department of Biotechnology, School of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu, 600119, India

    Antony V. Samrot, N. Shobana & G. Narendrakumar

  2. Department of Biomedical Sciences, Faculty of Medicine and Biomedical Sciences, MAHSA University, Jalan SP2, Bandar Saujana Putra, 42810, Jenjarom, Selangor, Malaysia

    Antony V. Samrot

  3. Department of Parasitology, Universiti Putra Malaysia, UPM Serdang, 43400, Seri Kembangan, Selangor, Malaysia

    S. Suresh Kumar

  4. Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang, 43400, Seri Kembangan, Selangor, Malaysia

    S. Suresh Kumar

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  1. Antony V. Samrot
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  2. N. Shobana
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Samrot, A.V., Shobana, N., Suresh Kumar, S. et al. Production, Optimization and Characterisation of Chitosanase of Bacillus sp. and its Applications in Nanotechnology. J Clust Sci 30, 607–620 (2019). https://doi.org/10.1007/s10876-019-01520-z

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  • DOI: https://doi.org/10.1007/s10876-019-01520-z

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