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Tissue engineering scaffolds of mesoporous magnesium silicate and poly(ε-caprolactone)–poly(ethylene glycol)–poly(ε-caprolactone) composite

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Abstract

Mesoporous magnesium silicate (m-MS) and poly(ε-caprolactone)–poly(ethylene glycol)–poly(ε-caprolactone) (PCL–PEG–PCL) composite scaffolds were fabricated by solvent-casting and particulate leaching method. The results suggested that the incorporation of m-MS into PCL–PEG–PCL could significantly improve the water adsorption of the m-MS/PCL–PEG–PCL composite (m-MPC) scaffolds. The in vitro degradation behavior of m-MPC scaffolds were determined by testing weight loss of the scaffolds after soaking into phosphate buffered saline (PBS), and the result showed that the degradation of m-MPC scaffolds was obviously enhanced by addition of m-MS into PCL–PEG–PCL after soaking for 10 weeks. Proliferation of MG63 cells on m-MPC was significantly higher than MPC scaffolds at 4 and 7 days. ALP activity on the m-MPC was obviously higher than MPC scaffolds at 7 days, revealing that m-MPC could promote cell differentiation. Histological evaluation showed that the introduction of m-MS into PCL–PEG–PCL enhanced the efficiency of new bone formation when the m-MPC scaffolds implanted into bone defect of rabbits. The results suggested that the inorganic/organic composite of m-MS and PCL–PEG–PCL scaffolds exhibited good biocompatibility, degradability and osteogenesis.

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Acknowledgments

This study was supported by grants from the National Natural Science Foundation of China (No. 81000799, 81271705, 31100680 and 51173041), Nano special program of Science and Technology Development of Shanghai (No. 12nm0500400), and the Key Medical Program of Science and Technology Development of Shanghai (No. 12441902800, 12441903600).

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Authors and Affiliations

  1. Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai, 200433, People’s Republic of China

    Dawei He, Xiaojiang Gu, Ming Li & Yunfei Niu

  2. Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Songchao Tang, Jie Wei & Zhenghui Liu

  3. Faculty of Health Service, Second Military Medical University, Shanghai, 200433, People’s Republic of China

    Wei Dong

  4. Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, People’s Republic of China

    Han Guo

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  1. Dawei He
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  2. Wei Dong
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  4. Jie Wei
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  7. Ming Li
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  9. Yunfei Niu
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Correspondence to Ming Li or Yunfei Niu.

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Wei Dong is the co-first author.

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He, D., Dong, W., Tang, S. et al. Tissue engineering scaffolds of mesoporous magnesium silicate and poly(ε-caprolactone)–poly(ethylene glycol)–poly(ε-caprolactone) composite. J Mater Sci: Mater Med 25, 1415–1424 (2014). https://doi.org/10.1007/s10856-014-5183-7

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  • DOI: https://doi.org/10.1007/s10856-014-5183-7

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