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Calcium alginate/dextran methacrylate IPN beads as protecting carriers for protein delivery

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Abstract

In the present study, mechanical and protein delivery properties of a system based on the interpenetration of calcium-alginate (Ca-Alg) and dextran-methacrylate (Dex-MA) networks are shown. Interpenetrated hydrogels beads were prepared by means of the alginate chains crosslinking with calcium ions, followed by the exposure to UV light that allows the Dex-MA network formation. Optical microscope analysis showed an average diameter of the IPN beads (Ca-Alg/Dex-MA) of 2 mm. This dimension was smaller than that of Ca-Alg beads because of the Dex-MA presence. Moreover, the strength of the IPN beads, and of their corresponding hydrogels, was influenced by the Dex-MA concentration and the crosslinking time. Model proteins (BSA and HRP) were successfully entrapped into the beads and released at a controlled rate, modulated by changing the Dex-MA concentration. The enzymatic activity of HRP released from the beads was maintained. These novel IPN beads have great potential as protein delivery system.

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Authors and Affiliations

  1. Department of Drug Chemistry and Technologies, “Sapienza” University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy

    Giorgia D’Arrigo, Chiara Di Meo, Laura Pescosolido, Tommasina Coviello, Franco Alhaique & Pietro Matricardi

Authors
  1. Giorgia D’Arrigo
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  2. Chiara Di Meo
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  3. Laura Pescosolido
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  4. Tommasina Coviello
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  5. Franco Alhaique
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  6. Pietro Matricardi
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Correspondence to Pietro Matricardi.

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D’Arrigo, G., Di Meo, C., Pescosolido, L. et al. Calcium alginate/dextran methacrylate IPN beads as protecting carriers for protein delivery. J Mater Sci: Mater Med 23, 1715–1722 (2012). https://doi.org/10.1007/s10856-012-4644-0

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  • DOI: https://doi.org/10.1007/s10856-012-4644-0

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