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Improved adherence and spreading of Saos-2 cells on polypropylene surfaces achieved by surface texturing and carbon nitride coating

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Abstract

The adhesion and contact guidance of human primary osteogenic sarcoma cells (Saos-2) were characterized on smooth, microstructured (MST) and micro- and nano-structured (MNST) polypropylene (PP) and on the same samples with a silicon-doped carbon nitride (C3N4-Si) coating. Injection molding was used to pattern the PP surfaces and the coating was obtained by using ultra-short pulsed laser deposition (USPLD). Surfaces were characterized using atomic force microscopy and surface energy components were calculated according to the Owens-Wendt model. The results showed C3N4-Si coated surfaces to be significantly more hydrophilic than uncoated ones. In addition, there were 86% more cells in the smooth C3N4-Si coated PP compared to smooth uncoated PP and 551%/476% more cells with MST/MNST C3N4-Si coated PP than could be obtained with MST/MNST uncoated PP. Thus the adhesion, spreading and contact guidance of osteoblast-like cells was effectively improved by combining surface texturing and deposition of osteocompatible C3N4-Si coating.

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Acknowledgments

This study was supported by the PhD-programme in Musculoskeletal Diseases and Biomaterials, Joint Research Program in Materials Science of Kuopio and Joensuu Universities and the Otto A. Malm Foundation. The authors thank Picodeon Ltd Oy, Helsinki, Finland for providing Coldab™ thin film depositions. Ari Halvari and Mikko Laasanen from Microsensor Laboratory are thanked for AFM imaging. Laboratory assistant Sanna Miettinen, technical assistant Juhani Hakala and the staff of BioMater Centre from the University of Kuopio are acknowledged for their technical support. We also thank Ewen MacDonald for language editing.

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  1. Department of Physics, University of Kuopio, P.O. Box 1627, 70211, Kuopio, Finland

    Katja Myllymaa, Sami Myllymaa, Hannu Korhonen & Reijo Lappalainen

  2. Microsensor Laboratory, School of Engineering and Technology, Savonia University of Applied Sciences, P.O.Box 6, 70201, Kuopio, Finland

    Katja Myllymaa

  3. Department of Applied Biotechnology, University of Kuopio, P.O. Box 1627, 70211, Kuopio, Finland

    Mikko J. Lammi

  4. Biocenter Kuopio, University of Kuopio, P.O. Box 1627, 70211, Kuopio, Finland

    Mikko J. Lammi

  5. Department of Chemistry, University of Joensuu, P.O. Box 111, 80101, Joensuu, Finland

    Hanna Saarenpää, Mika Suvanto & Tapani A. Pakkanen

  6. Department of Biomedicine, Anatomy, University of Kuopio, P.O. Box 1627, 70211, Kuopio, Finland

    Virpi Tiitu

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Myllymaa, K., Myllymaa, S., Korhonen, H. et al. Improved adherence and spreading of Saos-2 cells on polypropylene surfaces achieved by surface texturing and carbon nitride coating. J Mater Sci: Mater Med 20, 2337–2347 (2009). https://doi.org/10.1007/s10856-009-3792-3

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