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Anti-bacterial and cytotoxic properties of plasma sprayed silver-containing HA coatings

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Abstract

Silver-containing hydroxyapatite (HA) coatings have been prepared on titanium substrate by vacuum plasma spraying (VPS) method and anti-bacterial properties of the coatings were examined. Three types of bacteria stains, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus, were employed in this test. The results showed that the silver-containing HA coatings exhibited significant anti-bacterial effects against the three bacteria with anti-bacterial ratios higher than 95%. The release of silver ions in the physiological environment ensured excellent anti-bacterial properties of the silver-containing HA coatings. International standard ISO 10993-12 was adopted for cytotoxicity evaluation using fibroblast cell line L929, and it was found that the cytotoxicity for the coatings ranked 0 that showed no cytotoxicity for the coatings. Hemolysis test was processed according to ASTM F 756 standard with anti-coagulated rabbit blood, and the hemolysis ratios of the coatings were below 0.4%, indicating of non-hemolysis for the coatings.

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Authors and Affiliations

  1. Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, China

    Yikai Chen, Xuebin Zheng, Youtao Xie & Chuanxian Ding

  2. Shanghai Sixth People’s Hospital, Shanghai Jiaotong University, 600 Yishan Road, Shanghai, 200233, China

    Hongjiang Ruan & Cunyi Fan

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  1. Yikai Chen
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  2. Xuebin Zheng
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  3. Youtao Xie
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  4. Chuanxian Ding
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Correspondence to Xuebin Zheng.

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Chen, Y., Zheng, X., Xie, Y. et al. Anti-bacterial and cytotoxic properties of plasma sprayed silver-containing HA coatings. J Mater Sci: Mater Med 19, 3603–3609 (2008). https://doi.org/10.1007/s10856-008-3529-8

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  • DOI: https://doi.org/10.1007/s10856-008-3529-8

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