Abstract
An amidic derivative of carboxymethylcellulose was synthesized (CMCA). The new polysaccharide was obtained by converting a large percentage of carboxylic groups (∼50%) of carboxymethylcellulose into amidic groups rendering the macromolecule quite similar to hyaluronan. Then, the polysaccharide (CMCA) was crosslinked. The behavior of CMCA hydrogel towards normal human articular chondrocytes (NHAC) was in vitro studied monitoring the cell proliferation and synthesis of extra cellular matrix (ECM) components and compared with a hyaluronan based hydrogel (Hyal). An extracellular matrix rich in cartilage-specific collagen and proteoglycans was secreted in the presence of hydrogels. The injectability of the new hydrogels was also analysed. An experimental in vivo model was realized to study the effect of CMCA and Hyal hydrogels in the treatment of surgically created partial thickness chondral defects in the rabbit knee. The preliminary results pointed out that CMCA hydrogel could be considered as a potential compound for cartilage regeneration.
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Authors would like to thank Fondazione Monte dei Paschi di Siena (“Traslazione clinica attuali conoscenze precliniche nell’ambito dell’ingegneria tessutale muscolo scheletrica per ricostruzioni biologiche da perdite di sostanza traumatiche o patologiche” project) for financial support.
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Leone, G., Fini, M., Torricelli, P. et al. An amidated carboxymethylcellulose hydrogel for cartilage regeneration. J Mater Sci: Mater Med 19, 2873–2880 (2008). https://doi.org/10.1007/s10856-008-3412-7
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DOI: https://doi.org/10.1007/s10856-008-3412-7