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Protamine assisted rapid synthesis of carbon dots for living nucleolus imaging and gene delivery applications

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Abstract

The dynamics of the nucleolus in living cells are important in understanding cellular behavior and malignant transformation. However, construction of a fluorescent probe for long-term monitoring of nucleolus is significantly constrained by the cost, cellular barriers, cytotoxicity, and photobleaching. Herein, a one-pot microwave-assisted synthesis is developed for facile and quick preparation (domestic microwave oven, 7.5 min) of fluorescent carbon dots (CDs) for nucleolus imaging and tracking merely by adding protamine (PTM) into the precursor solution. The as-prepared carbon dots (CD-PTMs) feature photostability, biocompatibility, multicolor emission, and fluorescence enhancement upon binding with RNA. Significantly, CD-PTMs exhibit the capability of crossing both the cytoplasm and nuclear membranes, and located at the nucleolus for long-term (at least 12 h) imaging studies. Moreover, CD-PTMs are demonstrated to be capable of serving as efficient nanocarriers for in vitro transfection applications by taking advantage of the positively charged surface PTM derivative, showing the potential of CD-PTMs as multifunctional nanoprobes for the bioapplications.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (21906033 and 91643204), the introduced innovative R&D team project under the “The Pearl River Talent Recruitment Program” of Guangdong Province (2019ZT08L387), and National Key R&D Program of China (2016YFA0203103). We thank Haiyan Sui and Xiaoju Li from State Key Laboratory of Microbial Technology in Shandong University for NMR measurements.

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Authors and Affiliations

  1. Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Institute of Environmental Research At Greater Bay Area Ministry of Education, Guangzhou University, Guangzhou, 510006, People’s Republic of China

    Kena Zhang, Ge Ma, Zhenda Liang, Li Zhou & Bing Yan

  2. School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, People’s Republic of China

    Kena Zhang, Haiqing Wang & Bing Yan

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  1. Kena Zhang
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Correspondence to Li Zhou.

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Zhang, K., Ma, G., Wang, H. et al. Protamine assisted rapid synthesis of carbon dots for living nucleolus imaging and gene delivery applications. J Mater Sci 56, 4396–4406 (2021). https://doi.org/10.1007/s10853-020-05526-1

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