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Synthesis fluorescent magnetic nanoparticles in a microchannel using the La Mer process and the characterization of their properties

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Abstract

This study presents a stable and controllable synthesis of fluorescent magnetic nanoparticles in a flow-through microchannel for the bimodal use of magnetic activated cells sorting and fluorescence-activated cell sorters. The La Mer process is carried out to synthesize magnetic nanoparticles using co-precipitation. Then, the magnetic nanoparticles are coated with conjugation of chitosan and fluorescent isothiocyanate with two different concentrations. The chemical composition of the magnetic nanoparticles is determined by comparing the standard X-ray diffraction peaks of Fe3O4, and their sizes are also examined by using field emission scanning electron microscopy and dynamic light scattering measurement. The magnetic property of saturation magnetization and coercive field is characterized in a vibrating sample magnetometer. Also, the possibility of external manipulation in the synthesis of the magnetic particles is demonstrated by separating the synthesized fluorescent magnetic nanoparticles into a non-reacting lamination flow. Finally, their fluorescence property is determined by measuring the fluorescence adsorption spectra and the photoluminescence emission spectra in UV–Vis spectroscopy.

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References

  1. Pankhurst QA, Connolly J, Jones SK, Dobson J (2003) Applications of magnetic nanoparticles in biomedicine. J Phys D Appl Phys 36:R167–R181

    Article  Google Scholar 

  2. Colombo M, Romero SC, Casula MF, Gutiérrez L, Morales MP, Böhm IB, Heverhagen JT, Prosperi D, Parak WJ (2012) Biological applications of magnetic nanoparticles. Chem Soc Rev 41:4306–4334

    Article  Google Scholar 

  3. Gupta AK, Gupta M (2005) Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 26:3995–4021

    Article  Google Scholar 

  4. Gupta AK, Naregalkar RR, Vaidya VD, Gupta M (2007) Recent advances on surface engineering of magnetic iron oxide nanoparticles and their biomedical applications. Nanomedicine 2(1):23–39

    Article  Google Scholar 

  5. McCarthy JR, Kelly KA, Sun EY, Weissleder R (2007) Targeted delivery of multifunctional magnetic nanoparticles. Nanomedicine 2(2):153–167

    Article  Google Scholar 

  6. Duguet E, Vasseur S, Mornet S, Devoisselle JM (2006) Magnetic nanoparticles and their applications in medicine. Nanomedicine 1(2):157–168

    Article  Google Scholar 

  7. Koh I, Josephson L (2009) Magnetic nanoparticle sensors. Sensors 9:8130–8145

    Article  Google Scholar 

  8. Tartaj P, Morales MP, Veintemillas-Verdaguer S, Teresita G, Carreno T, Serna CJ (2003) The preparation of magnetic nanoparticles for applications in biomedicine. J Phys D Appl Phys 36:R182–R197

    Article  Google Scholar 

  9. Miltenyi S, Muller W, Weichel W, Radbruch A (2003) High gradient magnetic cell separation with MACS. Cytometry 11:231–238

    Article  Google Scholar 

  10. Dobson J (2008) Remote control of cellular behaviour with magnetic nanoparticles. Nat Nanotechnol 3:139–143

    Article  Google Scholar 

  11. Dulk RC, Schmidt KA, Sabatté G, Liébana S, Prins MWJ (2013) Magneto-capillary valve for integrated purification and enrichment of nucleic acids and proteins. Lab Chip 13(1):106–118

    Article  Google Scholar 

  12. Fu AY, Spence C, Scherer A, Arnold FH, Quake SR (1999) A microfabricated fluorescence-activated cell sorter. Nat Biotechnol 17:1109–1111

    Article  Google Scholar 

  13. Johnson KW, Dooner M, Quesenberry PJ (2007) Fluorescence activated cell sorting: a window on the stem cell. Curr Pharm Biotechnol 8(3):133–139

    Article  Google Scholar 

  14. Geens M, Velde HV, Block GD, Goossens E, Steirteghem AV, Tournaye H (2006) Spermatogonial survival after grafting human testicular tissue to immunodeficient mice. Hum Reprod 22(2):390–396

    Google Scholar 

  15. Corr SA, Rakovich YP, Gun’ko YK (2008) Multifunctional magnetic-fluorescent nanocomposites for biomedical applications. Nanoscale Res Lett 3:87–104

    Article  Google Scholar 

  16. Hatanaka S, Matsushita N, Abe M, Nishimura K, Hasegawa M, Handa H (2003) Direct immobilization of fluorescent dyes onto ferrite nanoparticles during their synthesis from aqueous solution. J Appl Phys 93:7569–7570

    Article  Google Scholar 

  17. Wang D, He J, Rosenzweig N, Rosenzweig Z (2004) Superparamagnetic Fe2O3 beads-CdSe/ZnS quantum dots core-shell nanocomposite particles for cell separation. Nano Lett 4:409–413

    Article  Google Scholar 

  18. Ge Y, Zhang Y, He S, Nie F, Teng G, Gu N (2009) Fluorescence modified chitosan-coated magnetic nanoparticles for high-efficient cellular imaging. Nanoscale Res Lett 4(4):287–295

    Article  Google Scholar 

  19. LaMer VK, Dinegar RH (1950) Theory, production and mechanism of formation of monodispersed hydrosols. J Am Chem Soc 72:4847–4854

    Article  Google Scholar 

  20. Tran LD, Hoang NMT, Mai TT, Tran HV, Nguyen NT, Tran TD, Do MH, Nguyen QT (2010) Nanosized magnetofluorescent Fe3O4–curcumin conjugate for multimodal monitoring and drug targeting. Colloids Surf A 371:104–112

    Article  Google Scholar 

  21. Kim HH, Kim EY, Park JC, Chang SC, Park YJ, Morten K, Go JS (2012) Continuous and surfactant-free preparation of nanocapsulized proteins. Microfluid Nanofluid 13(1):141–149

    Article  Google Scholar 

  22. Abraham S, Jeong EH, Arakawa T, Shoji S, Kim KC, Kim I, Go JS (2006) Microfluidics assisted synthesis of well-defined spherical polymeric microcapsules and their utilization as potential encapsulants. Lab Chip 6:752–756

    Article  Google Scholar 

  23. Valencia PM, Farokhzad OC, Karnik R, Langer R (2012) Microfluidic technologies for accelerating the clinical translation of nanoparticles. Nat Nanotechnol 7:623–629

    Article  Google Scholar 

  24. Marre S, Jensen F (2010) Synthesis of micro and nanostructures in microfluidic systems. Chem Soc Rev 39:1183–1202

    Article  Google Scholar 

  25. Jahn A, Reiner JE, Vreeland WN, DeVoe DL, Locascio LE, Gaitan M (2008) Preparation of nanoparticles by continuous-flow microfluidics. J Nanopart Res 10:925–934

    Article  Google Scholar 

  26. Caruso F, Susha AS, Giresig M, Möhwald H (1999) Magnetic core-shell particles: preparation of magnetite multilayers on polymer latex microspheres. Adv Mater 11:950–953

    Article  Google Scholar 

  27. Voigt A, Buske N, Sukhorukov GB, Antipov AA, Leporatti S, Lichtenfeld H, Bämler H, Donath E, Möhwald H (2001) Novel polyelectrolyte multilayer micro- and nanocapsules as magnetic carriers. J Magn Magn Mater 225:59–66

    Article  Google Scholar 

  28. Wacker JB, Lignos I, Parashar VK, Gijs MAM (2012) Controlled synthesis of fluorescent silica nanoparticles inside microfluidic droplets. Lab Chip 12:3111–3116

    Article  Google Scholar 

  29. Krishna KS, Navin CV, Biswas S, Singh V, Ham K, Bovenkamp GL, Theegala CS, Miller JT, Spivey JJ, Kumar CSSR (2013) Milifluidics for time-resolved mapping of the growth of gold nanostructures. JACS 135:5450–5456

    Article  Google Scholar 

  30. Zhai Y, Liu F, Zhang Q, Gao G (2009) Synthesis of magnetite nanoparticle aqueous dispersions in an ionic liquid containing acrylic acid anion. Colloids Surf A Physicochem Eng Asp 332:98–102

    Article  Google Scholar 

  31. Tural B, Ozkan N, Volkan M (2009) Preparation and characterization of polymer coated superparamagnetic magnetite nanoparticle agglomerates. J Phys Chem Solids 70:860–866

    Article  Google Scholar 

  32. Feltin N, Pileni MP (1997) New technique for synthesizing iron ferrite magnetic nanosized particles. Langmuir 13:3927–3933

    Article  Google Scholar 

  33. Li Y, Yi R, Yan A et al (2009) Facile synthesis and properties of ZnFe2O4 and ZnFe2O4/polypyrrole core-shell nanoparticles. Solid State Sci 11(8):1319–1324

    Article  Google Scholar 

  34. Zhao C-X, He L, Qiao SZ, Middelberg APJ (2011) Nanoparticle synthesis in microreactor. Chem Eng Sci 66:1463–1479

    Article  Google Scholar 

  35. Krishna KS, Li Y, Li S, Kumar CSSR (2013) Lab-on-a-chip synthesis of inorganic nanomaterials and quantum dots for biomedical applications. Adv Drug Deliv Rev 65:1470–1495

    Article  Google Scholar 

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Acknowledgements

This study was financially supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. NRF-2011-0017011 and No. K20701002274-12E0100-05710).

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Authors and Affiliations

  1. School of Mechanical Engineering, Pusan National University, Busan, 609-735, South Korea

    Thi Ngoan Nguyen, Hyeong Jin Joen, Bong Hyeon Kwon, Hyeong Hoon Kim & Jeung Sang Go

  2. Vietnam Academy of Science and Technology, Hanoi, Vietnam

    Thi Ngoan Nguyen & Dai Lam Tran

  3. John Radcliffe Hospital, University of Oxford, Oxford, UK

    Karl Mortan

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  1. Thi Ngoan Nguyen
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  2. Hyeong Jin Joen
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  3. Bong Hyeon Kwon
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  4. Hyeong Hoon Kim
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  5. Dai Lam Tran
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  6. Karl Mortan
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  7. Jeung Sang Go
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Correspondence to Jeung Sang Go.

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Nguyen, T.N., Joen, H.J., Kwon, B.H. et al. Synthesis fluorescent magnetic nanoparticles in a microchannel using the La Mer process and the characterization of their properties. J Mater Sci 49, 4583–4589 (2014). https://doi.org/10.1007/s10853-014-8158-7

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  • DOI: https://doi.org/10.1007/s10853-014-8158-7

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