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Effect of salicylic acid on fatty acid accumulation in Phaeodactylum tricornutum during stationary growth phase

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Abstract

Microalgae possess great potential for use as raw materials for biofuel production. The marine diatom Phaeodactylum tricornutum accumulates lipids as its major form of energy storage when grown under specific conditions. This study investigated the effects of salicylic acid (SA) on the accumulation of fatty acids in P. tricornutum in the stationary growth phase. Our results showed that 40 μM SA increased the total fatty acid content accumulation 1.3-fold of the control after 4 days of exposure. The metabolomics data indicated that SA stimulated the fatty acid monostearin, 2-monoolein, monoolein, and methyl palmitoleate to 22.7-, 16.3-, 3.47-, and 3.47-fold of the control, respectively. The results of physiological and gene transcriptional levels revealed that the glycolysis, N assimilation, and fatty acid synthesis pathways were induced by SA treatment and thus accelerated fatty acid synthesis from carbohydrate and N compounds. The SA treatment could be used to optimize biodiesel production.

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Acknowledgments

This work was financially supported by the Natural Science Foundation of China (21577128).

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Authors and Affiliations

  1. Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, 310032, People’s Republic of China

    Jiahui Xu, Xingxing Li & Guanfu Liu

  2. College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, People’s Republic of China

    Xiaoji Fan & Zhengwei Fu

  3. College of Environment, Zhejiang University of Technology, Hangzhou, 310032, People’s Republic of China

    Zhenyan Zhang, Youchao Zhu & Haifeng Qian

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  1. Jiahui Xu
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  2. Xiaoji Fan
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Correspondence to Haifeng Qian.

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Xu, J., Fan, X., Li, X. et al. Effect of salicylic acid on fatty acid accumulation in Phaeodactylum tricornutum during stationary growth phase. J Appl Phycol 29, 2801–2810 (2017). https://doi.org/10.1007/s10811-017-1191-6

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  • DOI: https://doi.org/10.1007/s10811-017-1191-6

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