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Effect of enzyme-assisted extract of Sargassum coreanum on induction of apoptosis in HL-60 tumor cells

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Abstract

In order to effectively prepare useful components from Sargassum coreanum, enzyme-assisted extraction was adapted to extraction processing of the seaweed. Our previous studies have shown that Neutrase extract of S. coreanum exhibited the strongest antioxidant activities among ten enzymatic extracts. It is reported that many antioxidant substances have anticancer or anticarcinogenic properties. Thus, Neutrase extract, which possessed a strong antioxidant activity in the previous study, was separated into four different molecular weight fractions (<5, 5–10, 10–30, and >30 kDa) which were screened for inhibitory activities against cancer cell growth. The >30-kDa fraction inhibited cell growth more than the other fractions. We separated crude polysaccharide (CPS) from the >30-kDa fraction to show that CPS increased DNA fragmentation, apoptotic body, and apoptotic cells with hypodiploid DNA contents in HL-60 cells. This indicates that CPS suppressed the growth of cells through apoptosis. The CPS gradually increased the expression of pro-apoptotic Bax and led to the activation of caspase-3 and cleavage of poly(ADP-ribose) polymerase. The CPS was 40.85% fucose, 27.24% galactose, and 12.99% glucose, as analyzed by bio-liquid chromatography. These findings suggest that apoptosis effect can be mediated by CPS in HL-60 cells.

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Acknowledgment

This research was supported by a grant from the Jeju National University fund, Korea.

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Authors and Affiliations

  1. Department of Marine Life Science, Jeju National University, Jeju, 690-756, South Korea

    Seok-Chun Ko, Seung-Hong Lee, Ginnae Ahn, Ki-Wan Lee & You-Jin Jeon

  2. Jeju Biodiversity Research Institute (JBRI), Jeju Hi-Tech Industry Development Institute (HiDI), Jeju, 697-943, South Korea

    Kil-Nam Kim

  3. Marine Bio Research Team, Korea Basic Science Institute, Jeju, 690-756, South Korea

    Seon-Heui Cha

  4. Department of Chemistry, Pukyoung National University, Busan, 608-737, South Korea

    Se-Kwon Kim

  5. Korean Nokyong Research Center, College of Medicine Konkuk University, Chungju, 380-701, South Korea

    Byong-Tae Jeon

  6. Department of Biotechnology, College of Medicine Konkuk University, 322 Danwol-dong, Chungju, 380-701, South Korea

    Pyo-Jam Park

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  1. Seok-Chun Ko
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Ko, SC., Lee, SH., Ahn, G. et al. Effect of enzyme-assisted extract of Sargassum coreanum on induction of apoptosis in HL-60 tumor cells. J Appl Phycol 24, 675–684 (2012). https://doi.org/10.1007/s10811-011-9685-0

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  • DOI: https://doi.org/10.1007/s10811-011-9685-0

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