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Titanium Ions Play a Synergistic Role in the Activation of NLRP3 Inflammasome in Jurkat T Cells

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Abstract

Release of titanium ions (Ti ions) frequently occurs around dental implants and, as a consequence, higher content of Ti is typically present in peri-implantitis tissue. Unlike chronic periodontitis, Ti ions may play a role in the development of peri-implantitis. Inflammasomes are multiprotein signal transduction complexes, involved in inflammation and immune response, which lead to the secretion of mature cytokines associated with the progression of peri-implantitis. It is well known that T lymphocytes dominate the immune response in peri-implantitis, but whether Ti ions can impact the assembly of functional inflammasomes in T cells still remains unclear. Here, we observed that the mRNA expression of NLRP3 and CASP1, as well as the secretion of IL-1β, increased after 6-h incubation of Jurkat T cells with PHA and Ti ions. Moreover, measurement by confocal microscopy and flow cytometry assay indicates that Ti ions can promote the production of ROS, while NLRP3 expression and IL-1β secretion are reduced after treatment of Jurkat cells with NAC (ROS scavenger). Taken together, we presently show that Ti ions can activate NLRP3 inflammasome and then promote IL-β secretion in vitro, where ROS may play a mechanistic role in this activation process.

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Abbreviations

NAC:

N-acetyl cysteine

PHA:

phytohaemagglutinin

NLRP1:

NLR family, pyrin domain containing 1

AIM2:

absent in melanoma 2

NLRP3:

NLR family, pyrin domain containing 3

NLRC4:

NLR family CARD domain-containing protein 4

ASC:

apoptosis-associated speck-like protein containing a CARD

DCF:

2′,7′-dichlorodihydrofluorescein

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Funding

This study was supported by the National Natural Science Foundation of China (Grant 81460108).

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Authors and Affiliations

  1. Department of Implant Dentistry, Stomatology Hospital, Guangxi Medical University, 10 Shuangyong Rd, Nanning, Guangxi, China

    Xiao Li, Li Tang &  Ye Myat Thu

  2. Oral Medicine & Periodontology, Stomatology Hospital, Guangxi Medical University, Nanning, Guangxi, China

    Donghui Chen

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Correspondence to Li Tang.

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Li, X., Tang, L., Ye Myat Thu et al. Titanium Ions Play a Synergistic Role in the Activation of NLRP3 Inflammasome in Jurkat T Cells. Inflammation 43, 1269–1278 (2020). https://doi.org/10.1007/s10753-020-01206-z

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