Abstract
Chlorogenic acid (CGA), a polyphenolic compound, exists widely in medicinal herbs, which has been shown a strong antioxidant and anti-inflammatory effect. This study investigated the protective effects and mechanism of CGA on lipopolysaccharide (LPS)-induced acute kidney injury (AKI). Treatment of CGA successfully ameliorates LPS-induced renal function and pathological damage. Moreover, CGA dose-dependently suppressed LPS-induced blood urea nitrogen (BUN), creatinine levels, and inflammatory cytokines TNF-α, IL-6, and IL-1β in serum and tissue. The relative proteins’ expression of TLR4/NF-κB signal pathway was assessed by western blot analysis. Our results showed that CGA dose-dependently attenuated LPS-induced kidney histopathologic changes, serum BUN, and creatinine levels. CGA also suppressed LPS-induced TNF-α, IL-6, and IL-1β production both in serum and kidney tissues. Furthermore, our results showed that CGA significantly inhibited the LPS-induced expression of phosphorylated NF-κB p65 and IκB as well as the expression of TLR4 signal. In conclusion, our results provide a mechanistic explanation for the anti-inflammatory effects of CGA in LPS-induced AKI mice through inhibiting TLR4/NF-κB signaling pathway.
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He, S., X. Li, R. Li, L. Fang, L. Sun, Y. Wang, and M. Wu. 2016. Annexin A2 modulates ROS and impacts inflammatory response via IL-17 signaling in polymicrobial sepsis mice. PLoS Pathogens 12: e1005743.
Mårtensson, J., and R. Bellomo. 2016. Pathophysiology of septic acute kidney injury. Contributions to Nephrology 187: 36–46.
Wang, C., H. Sun, Y. Song, et al. 2015. Pterostilbene attenuates inflammation in rat heart subjected to ischemia-reperfusion: role of TLR4/NF-κB signaling pathway. International Journal of Clinical and Experimental Medicine 8: 1737–1746.
Hu, J., and L. Liu. 2016. Licochalcone A attenuates lipopolysaccharide-induced acute kidney injury by inhibiting NF-κB activation. Inflammation 39: 569–574.
Relja, B., E. Töttel, L. Breig, et al. 2012. Plant polyphenols attenuate hepatic injury after hemorrhage/resuscitation by inhibition of apoptosis, oxidative stress, and inflammation via NF-kappaB in rats. European Journal of Nutrition 51: 311–321.
Tsumbu, C.N., G. Deby-Dupont, M. Tits, et al. 2012. Polyphenol content and modulatory activities of some tropical dietary plant extracts on the oxidant activities of neutrophils and myeloperoxidase. International Journal of Molecular Sciences 13: 628–650.
Tuñón, M.J., M.V. García-Mediavilla, and S. Sánchez-Campos. 2009. Potential of flavonoids as anti-inflammatory agents: modulation of pro-inflammatory gene expression and signal transduction pathways. Current Drug Metabolism 10: 256–271.
Maqsood, S., and S. Benjakul. 2010. Comparative studies of four different phenolic compounds on in vitro antioxidative activity and the preventive effect on lipid oxidation of fish oil emulsion and fish mince. Food Chemistry 119: 123–132.
Bhoyar, M.S., G.P. Mishra, P.K. Naik, et al. 2011. Estimation of antioxidant activity and total phenolics among natural populations of Caper (Capparis spinosa) leaves collected from cold arid desert of trans-Himalayas. Australian Journal of Crop Science 5: 912–919.
Leal, C.A., D.B. Leal, S.A. Adefegha, and Morsch. 2016. Effects of chlorogenic acid on adenine nucleotides hydrolyzing enzyme activities and expression in platelets of rats experimentally demyelinated with ethidium bromide. Biomedicine & Pharmacotherapy 81: 363–370.
Zhang, X., H. Huang, T. Yang, et al. 2010. Chlorogenic acid protects mice against lipopolysaccharide-induced acute lung injury. Injury 41: 746–752.
Ye, H.Y., Z.Y. Li, Y. Zheng, et al. 2016. The attenuation of chlorogenic acid on oxidative stress for renal injury in streptozotocin-induced diabetic nephropathy rats. Archives of Pharmacal Research 39: 989–997.
Schaalan, M.F., and W.A. Mohamed. 2016. Determinants of hepcidin levels in sepsis-associated acute kidney injury: Impact on pAKT/PTEN pathways? Journal of Immunotoxicology 13: 751–757.
Wu, Y., Y. Zhang, L. Wang, et al. 2015. The role of autophagy in kidney inflammatory injury via the NF-κB route induced by LPS. International Journal of Medical Sciences 12: 655–667.
Feng, Y., Y.H. Yu, S.T. Wang, et al. 2016. Chlorogenic acid protects d-galactose-induced liver and kidney injury via antioxidation and anti-inflammation effects in mice. Pharmaceutical Biology 54: 1027–1034.
Chen, Y., Y. Du, Y. Li, et al. 2015. Panaxadiol saponin and dexamethasone improve renal function in lipopolysaccharide-induced mouse model of acute kidney injury. PLoS One 10: e0134653.
Mortensen, J., B. Shames, C.P. Johnson, et al. 2011. MnTMPyP, a superoxide dismutase/catalase mimetic, decreases inflammatory indices in ischemic acute kidney injury. Inflammation Research 60: 299–307.
Xu, D., M. Chen, X. Ren, et al. 2014. Leonurine ameliorates LPS-induced acute kidney injury via suppressing ROS-mediated NF-κB signaling pathway. Fitoterapia 97: 148–155.
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All animal protocols were approved by the Institutional Animal Care and Use Committee at Wenzhou Medical University and were consistent with the Guide for the Care and Use of Laboratory Animals (updated (2011) version of the NIH guidelines.
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Ye, HY., Jin, J., Jin, LW. et al. Chlorogenic Acid Attenuates Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting TLR4/NF-κB Signal Pathway. Inflammation 40, 523–529 (2017). https://doi.org/10.1007/s10753-016-0498-9
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DOI: https://doi.org/10.1007/s10753-016-0498-9