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Variations in kinetics of alkaline phosphatase in sediments of eutrophic, shallow, Chinese lakes

  • Eutrophication in Lakes
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Abstract

Kinetics of alkaline phosphatase in sediments of a shallow Chinese freshwater lake (Lake Donghu) were investigated. Spatially, among 20 sites sampled, V max and K m values of alkaline phosphatase in surface sediments were higher in the zone adjacent to sites with the highest chlorophyll a concentrations. Vertically, there was a peak in V max at intermediate sediment depths in addition to the expected maximum at the surface. Some inhibitors, such as CuSO4, ZnSO4 and Na2WO4, showed significantly different effects on kinetics of alkaline phosphatase in interstitial water and sediments. Moreover, alkaline phosphatase in interstitial water and sediments responded to Na2WO4 in different ways in Lake Taihu. These observations imply that the enzyme is immobilized in sediments, which became more stable with accelerated eutrophication, as suggested by highest alkaline phosphatase activity (APA) in sediments corresponding with highest water column chlorophyll a concentrations in Lake Donghu.

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Acknowledgements

This work was supported by the grant (KZCX1-SW-12-II-02-02) from the Chinese Academy of Sciences (CAS), National Basic Research Program of China (2002CB412300) and funding from the National Science Foundation of China (39170165, 39670149 and 20177033). We also obliged to the funds (2002AA601013). The authors also gratefully acknowledge the support of the Donghu Lake Ecosystem Experimental Station of CAS for the help in sampling collection.

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Authors and Affiliations

  1. Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan, 430072, China

    Yiyong Zhou, Xiuyun Cao, Chunlei Song, Jianqiu Li, Guoyuan Chen & Liang Peng

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China

    Xiuyun Cao, Chunlei Song, Guoyuan Chen & Liang Peng

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  1. Yiyong Zhou
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  2. Xiuyun Cao
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  3. Chunlei Song
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  4. Jianqiu Li
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  5. Guoyuan Chen
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  6. Liang Peng
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Correspondence to Yiyong Zhou.

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Zhou, Y., Cao, X., Song, C. et al. Variations in kinetics of alkaline phosphatase in sediments of eutrophic, shallow, Chinese lakes. Hydrobiologia 581, 109–116 (2007). https://doi.org/10.1007/s10750-006-0503-7

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