Abstract
The miR167 family has been shown to play roles in regulating plant fertility by control the expression of a set of ARF transcription factors. In this study, the precursor of miR167a was cloned from the photo–thermo-sensitive genic male sterile (PTGMS) wheat line BS366. Moreover, we identified the target gene, TaARF8, from PTGMS wheat line BS366, and used the 5′-RACE assay to confirm the cleavage site. Overexpression (OE) of TaemiR167a in Arabidopsis induced male sterility phenotypes, and reduced the expression levels of AtARF6 and AtARF8. Meanwhile, OE of TaemiR167a reduced the content of endogenous jasmonic acid (JA) and indole acetic acid (IAA) in transgenic Arabidopsis lines. In PTGMS wheat line BS366, the expression levels of TaemiR167a were up-regulated in anther tissues during pollen development process, while the transcriptional levels of TaARF8 were down-regulated under sterile conditions. Importantly, TaemiR167a was sensitive to low temperature (10°), and was highly expressed in anther tissues of BS366 at pollen developmental stage 3 in sterile environment. Then, we measured the endogenous JA and IAA in BS366 anther tissues, and found that the accumulation of JA and IAA in anther was abnormal during the pollen development process. These results suggested that TaemiR167a-mediated regulation of TaARF8 and changes of endogenous JA and IAA during pollen developmental process took part in the pollen fertility control in PTGMS wheat line BS366.
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We gratefully thank the editor’s efficient works and the good comments and suggestions from two reviewers. This work is supported by National Natural Science Foundation of China (No. 31872881), Beijing Municipal Natural Science Foundation (No. 6182014), BAAFS Science and Technology Innovation Foundation (No. KJCX20180403) and National Key Research Project (No. 2016YFD0101601).
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Supplementary material 1 (TIFF 3417 kb) Fig. S1 Analysis of TaemiR167a sequence and structure. a ClustalX nucleic acid sequence alignment between TaemiR167a and the miR167a from Arabidopsis thaliana (At, miRBase accession number MI0000208), Oryza sativa (Os, miRBase accession number MI0000676), Zea mays (Zm, miRBase accession number MI0001475), Sorghum bicolor (Sb, miRBase accession number MI0001513), Glycine max (Gm, miRBase accession number MI0001777). Green values in brackets represented the precursor sequence length of each miR167a and b the secondary structure analysis of six precursor sequences of different miR167a
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Supplementary material 2 (TIFF 6223 kb) Fig. S2 Characteristics of TaARF8. a PCR productions of TaARF8-A and -D. M DNA ladder, b the protein sequence alignment of TaARF8-A and TaARF8-D. Red line indicated B3 DNA binding motif, blue line indicated auxin response domain, and green line indicated AUX/IAA domain and c a phylogenetic tree of TaARF8, OsARF8, ZmARF8 and AtARF6 and eight proteins. Os rice, Zm Zea mays, At Arabidopsis
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Supplementary material 3 (TIFF 933 kb) Fig. S3 Identification of TaemiR167a cleavage site using the 5’-RACE. a PCR production of 3’-TaARF8 and b the region targeted by TaemiR167a in TaARF8 nucleotide sequence
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Supplementary material 4 (TIFF 1963 kb) Fig. S4 The expression patterns of TaemiR167a and its target gene TaARF8 during the stage of pollen developmental process, and the measurement of endogenous JA and IAA. a The expression patterns of TaemiR167a in Jing411 and BS366 anther tissues from pollen developmental stages 1 to 3. Bars indicated means ± SD (n = 3). Black star indicted the significant difference between stages 2 or 3 and 1. *p < 0.05,**p < 0.01, Student’s t-test, b the expression patterns of TaARF8 in Jing411 and BS366 anther tissues from pollen developmental stages 1 to 3. Bars indicated means ± SD (n = 3). Black star indicted the significant difference between stages 2 or 3 and 1. *p < 0.05, **p < 0.01, Student’s t-test, c the content of endogenous JA in Jing411 and BS366 anther tissues from pollen developmental stages 1 to 3. Bars indicated means ± SD (n = 3). Black star indicted the significant difference between stages 2 or 3 and 1. *p < 0.05, Student’s t-test. Black star displayed the significant difference between stages 2 or 3 and 1 and d the concentration of endogenous IAA in Jing411 and BS366 anther tissues from pollen developmental stages 1 to 3. Bars indicated means ± SD (n = 3). Black star indicted the significant difference betweens stage 2 or 3 and 1. *p < 0.05, Student’s t-test. Black star displayed the significant difference between stages 2 or 3 and 1
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Wang, Y., Duan, W., Bai, J. et al. Constitutive expression of a wheat microRNA, TaemiR167a, confers male sterility in transgenic Arabidopsis. Plant Growth Regul 88, 227–239 (2019). https://doi.org/10.1007/s10725-019-00503-4
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DOI: https://doi.org/10.1007/s10725-019-00503-4