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Histone acetylation modification affects cell wall degradation and aerenchyma formation in wheat seminal roots under waterlogging

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Abstract

Recent studies have shown that histone acetylation, which is an important epigenetic modification, plays a key role in abiotic stress responses in plants. Programmed cell death and aerenchyma formation occur in cortical cells of wheat seminal roots under waterlogging stress. To explore the role of histone acetylation in aerenchyma formation of cortical cells under waterlogging, the seminal roots of two wheat cultivars, namely, Huamai 8 (waterlogging-tolerant) and Huamai 9 (waterlogging-sensitive) were investigated with waterlogging and simultaneous treatment with an acetylation inhibitor. In this study, the immunefluorescence technique and Western blotting were used to determine the histone acetylation levels in wheat seminal roots under waterlogging stress. Cell wall degradation-related enzymes (cellulase and pectinase) were observed using the method of ultracytochemical localization. We also tested the expression of related genes, such as histone acetyltransferase (HAT1), histone deacetylase (HD3), endoglucanase (CEL), polygalacturonase (PG), and xyloglucan endotransglucosylase (XET). The results indicated that histone acetylation is involved in eliciting responses to waterlogging stress as well as in the aerenchyma formation by affecting cell wall degradation of cortical cells in wheat seminal roots. We also present a model of waterlogging-induced aerenchyma formation in cortical cells of wheat seminal roots based on our experimental results and the findings of previous studies.

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Abbreviations

CCD:

Charge-coupled device

CEL:

Endoglucanase cellulase

HAT:

Histone acetyltransferase

HDAC:

Histone deacetylase

PAGE:

Polyacrylamide gel electrophoresis

PCD:

Programmed cell death

PCR:

Polymerase chain reaction

PG:

Polygalacturonase pectinase

SDS:

Sodium dodecyl sulfate

XET:

Xyloglucan endotransglycosylase

TEM:

Transmission electron microscopy

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Acknowledgements

This work was supported by the National Nature Science Foundation of China (Grant Nos. 31071347 and 31471428). We would like to thank Cao Jianbo and He Limin for providing the transmission electron microscope technology and the LetPub for providing linguistic assistance during the preparation of this manuscript.

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Authors and Affiliations

  1. Laboratory of Cell Biology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Chengyang Li, Dongcheng Liu, Ze Lin, Bin Guan, Dan Liu, Li Yang & Zhuqing Zhou

  2. College of Food & Biological Science and Technology, Wuhan Institute of Design and Sciences, Wuhan, 430070, Hubei, China

    Xiangyi Deng

  3. College of Plant Sciences & Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Fangzhu Mei

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  1. Chengyang Li
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  2. Dongcheng Liu
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  3. Ze Lin
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  4. Bin Guan
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  5. Dan Liu
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  6. Li Yang
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  9. Zhuqing Zhou
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Contributions

LC conceived and designed research. LC and LD conducted experiments. GB contributed new reagents or analytical tools. LD, YL and LZ analyzed data. LC wrote the manuscript under the help of DX and ZZ. All authors read and approved the manuscript.

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Correspondence to Zhuqing Zhou.

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Li, C., Liu, D., Lin, Z. et al. Histone acetylation modification affects cell wall degradation and aerenchyma formation in wheat seminal roots under waterlogging. Plant Growth Regul 87, 149–163 (2019). https://doi.org/10.1007/s10725-018-0460-y

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