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Plant growth-promoting potential of endophytic bacteria isolated from roots of wild Dodonaea viscosa L.

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Abstract

Dodonaea viscosa, a wild and perennial shrub that can tolerate harsh environmental conditions, was used for the isolation of its endophytic bacteria and their potential was explored for the promotion of Canola growth. The bacteria identified through 16S rRNA gene sequencing, belonged to ten different genera namely Inquilinus, Xanthomonas, Pseudomonas, Rhizobium, Brevundimonas, Microbacterium, Bacillus, Streptomyces, Agrococcus and Stenotrophomonas. All the strains produced small amount of IAA (indole acetic acid) in the absence of tryptophan and comparatively more in the presence of tryptophan. All the bacterial strains were positive for ammonia production, cellulase and pectinase activity, but few of them showed phosphate solubilization, siderophore and hydrogen cyanide production. Only three strains showed ACC (1-aminocyclopropane-1-carboxylate) deaminase activity when tested using in-vitro enzyme assay. Members of genera Bacillus, Pseudomonas and Streptomyces showed positive chitinase, protease and antifungal activity against two phytopathogenic fungi Aspergillus niger and Fusarium oxysoprum, while members of Xanthomonas, Pseudomonas and Bacillus showed significant root elongation of Canola which could be related with their positive plant-growth-promoting (PGP) traits. Among the three plant growth promoting Bacillus strains, B. idriensis is never reported before for its PGP activities. These results showed the potential of Dodonaea viscosa endophytic bacteria as PGPBs, which in future can be further explored for their host range/molecular mechanisms.

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Acknowledgments

The present work was funded by Pakistan Academy of Sciences. Imran Afzal was a recipient of a fellowship from Higher Education Commission of Pakistan. Funding was provided by National Natural Science Foundation of China (Grant No. ID0E5VAI1874) and Henan Agricultural Science and Technology Innovation Fund (CN) (Grant No. ID0ENDBI1892).

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Authors and Affiliations

  1. Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan

    Imran Afzal, Irum Iqrar & Zabta K. Shinwari

  2. Microbiology & Biotechnology Research Lab, Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi, Pakistan

    Azra Yasmin

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  1. Imran Afzal
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Correspondence to Azra Yasmin.

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Afzal, I., Iqrar, I., Shinwari, Z.K. et al. Plant growth-promoting potential of endophytic bacteria isolated from roots of wild Dodonaea viscosa L.. Plant Growth Regul 81, 399–408 (2017). https://doi.org/10.1007/s10725-016-0216-5

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